VEGF Bioassay Propagation Model Technical Manual
Instructions for Use of Product(s)
GA1082
Literature # TM545
The VEGF Bioassay, Propagation Model is a bioluminescent cell-based assay that measures VEGF stimulation and inhibition of KDR (VEGFR2) using the NFAT-RE as a readout. This assay overcomes many of the limitations of the current endothelial cell proliferation assays, and can be used for the discovery and development of novel biologic therapies aimed at either inducing or inhibiting the VEGF response. The KDR/NFAT-RE HEK293 Cells are provided in a Cell Propagation Model (CPM) format, which includes cryopreserved cells that can be thawed, propagated and banked for long-term use (also offered in a thaw-and-use format; Cat.# GA2001, GA2005).
The KDR/NFAT-RE HEK293 Cells have been engineered to express the NFAT response element upstream of Luc2P as well as exogenous KDR. When VEGF binds to the KDR/NFAT-RE HEK293 Cells, the KDR transduces intracellular signals resulting in NFAT-RE-mediated luminescence. The bioluminescent signal is detected and quantified using Bio-Glo™ Luciferase Assay System and a standard luminometer, such as the GloMax® Discover System.
Summary of Changes
The following changes were made to the 12/22 revision of this document:
1. In Section 4, Note “a.” the suggested starting quantity for test and reference samples was updated from 18μg/ml to 6μg/ml.
2. Disclaimers and Section 7.D, Related Products were updated. General formatting of other sections updated.
3. “Notes” were changed from numerical to alphabetical listing.
4. Template, TM cover and font were updated.
Revised 12/22.