ProDye® Terminator Sequencing System
Sanger Sequencing Chemistry for Capillary Electrophoresis Platforms
- Easily implemented with the same protocol, workflows and calibration
- Designed for a wide range of sequencing applications
- Provides enhanced productivity while reducing costs
Catalog Number:
Size
Catalog Number: CR4324
Catalog Number: CR4302
Catalog Number: CR4310
Sanger Sequencing in Perfect Harmony with Your Workflow
The ProDye® Terminator Sequencing System provides an improved method of fluorescent Sanger sequencing using a proprietary thermostable DNA polymerase included in the master mix. Compatible with a variety of DNA templates, the ProDye® Terminator Sequencing System can be used with the Spectrum Compact CE System as well as the Applied Biosystems® 3130, 3130xl, 3500, 3500xL, 3730, 3730xl and SeqStudio® Genetic Analyzers. The ProDye® Terminator Sequencing System uses dTMR, dCXR, dRSixG and dROneTen terminator dyes, the same dyes contained in the BigDye® Terminator V3.1 Cycle Sequencing Kit.
Your Sample, Our Sequencing Kit, Any Instrument
Integrate the ProDye® Terminator Sequencing System seamlessly into a familiar workflow.
Familiar Reaction Setup
Includes all the components for the sequencing reaction in a ready-to-use, premixed format—just add your template and the template-specific primer.
- Proprietary enzyme blend in 2.5X Master Mix
- Additional 5X Sequencing Buffer and Nuclease-Free Water is included
- Supports 10μl reactions and 20μl 0.5X reactions
Compatible with a Variety of Thermal Cyclers
Simple cycling protocol designed for obtaining maximum read lengths.
Compatible with a Variety of Samples and Running Conditions
Sequence plasmid DNA and other samples using 'Standard' or 'Fast' sequencing protocols.
Chromatograms from NIST plasmid ERCC-00083 cycle sequenced with ProDye® Terminator Sequencing System and detected on the Spectrum Compact CE System using the ‘Standard’ sequencing protocol. Plasmid ERCC00083 from NIST was cycle sequenced with ProDye® Terminator Sequencing System. Sequencing reactions were purified with ethanol/EDTA-based precipitation and denatured in Hi-Di™ Formamide before detection on the Spectrum Compact CE System using the T_Seq_36_Std protocol (Panel A) and T_Seq_36_Fast protocol (Panel B).
Chromatograms from control pGEM®-3Zf(+) plasmid cycle sequenced with ProDye® Terminator Sequencing System and detected on the Spectrum Compact CE System using the ‘Standard’ sequencing protocol. pGEM®-3Zf(+) plasmid DNA was cycle sequenced with ProDye® Terminator Sequencing System. Sequencing reactions were purified with ethanol/EDTA-based precipitation and denatured in Hi-Di™ Formamide before detection on the Spectrum Compact CE System using the T_Seq_36_Std protocol (Panel A) and T_Seq_36_Fast protocol (Panel B).
Sequencing Analysis Read Lengths
Combine ProDye® Terminator Sequencing System with the Spectrum Compact CE System for more bases in less time.
Chromatograms from control pGEM®-3Zf(+) plasmid cycle sequenced with ProDye® Terminator Sequencing System and detected on the Spectrum Compact CE System using T_Seq_36_Std protocol (Panel A) and T_Seq_36_Fast protocol (Panel B).
Product Application: Sanger Sequencing of SARS-CoV-2
Sequence a region of the SARS-CoV-2 genome using the ProDye® Terminator Sequencing System with analysis by capillary electrophoresis on the Spectrum Compact CE System.
ProView® Sequencing Software
ProView® Sequencing Software is a customizable Sanger sequencing viewer capable of displaying .ab1 files generated on many currently available capillary electrophoresis (CE) sequencers. This software can be used to view and edit sequence information, display electropherograms, generate reports, display embedded information and save edited files in multiple formats.
Comprehensive Technical Support
We understand that your work can’t stop and we are dedicated to minimizing interruptions to your lab workflow. Whether you are working with a new sample type, implementing Sanger sequencing on a new instrument, or anything in between, you can be confident that your sequencing will hit all the right notes with our comprehensive technical support. Promega scientists are ready to help, and we will be there when you need us.
Protocols
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
ProDye® 2.5X Master Mix |
CR100A | 1 × 200μl |
ProDye® 5X Sequencing Buffer |
CR101A | 1 × 1ml |
|
pGEM®-3Zf(+) Vector |
P227A | 1 × 20μg |
|
pUC/M13 Primer, Forward (24mer) |
Q560A | 1 × 2μg |
Nuclease-Free Water |
P119A-C | 1 × 1.25ml |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box
| Item | Part # | Choose a size | Available Separately |
|---|---|---|---|
ProDye® 2.5X Master Mix |
CR100B | 2 × 800μl | |
ProDye® 5X Sequencing Buffer |
CR101A | 3 × 1ml | |
|
pGEM®-3Zf(+) Vector |
P227A | 1 × 20μg | |
|
pUC/M13 Primer, Forward (24mer) |
Q560A | 1 × 2μg | |
|
Nuclease-Free Water |
P119A | 5 × 1,250μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box
| Item | Part # | Choose a size | Available Separately |
|---|---|---|---|
ProDye® 2.5X Master Mix |
CR100B | 10 × 800μl | |
ProDye® 5X Sequencing Buffer |
CR101B | 1 × 12ml | |
|
pGEM®-3Zf(+) Vector |
P227A | 1 × 20μg | |
|
pUC/M13 Primer, Forward (24mer) |
Q560A | 1 × 2μg | |
|
Nuclease-Free Water |
P119E | 2 × 13ml | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
Articles
- Product Application: ProDye™ System Reaction Cleanup with Wizard® MagneSil® Sequencing Reaction Clean-Up System
- Product Application: Spectrum Compact and ProDye™ System for Fish Authentication by Sanger Sequencing
- Product Application: Spectrum Compact and ProDye™ System for SARS-CoV-2 Sanger Sequencing
- Product Application: ProDye™ System Reaction Cleanup with EtOH in Tubes
- Product Application: Bacterial Authentication by Sanger Sequencing using ProDye™ System and Spectrum Compact
Related Products
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ProDye® 4C Matrix Standard
DNA fragments labeled with four different fluorescent dyes in one tube for use with the ProDye® Terminator Sequencing System.
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ProDye® 5X Sequencing Buffer
Optimized for use with the ProDye® Terminator Sequencing System.
CR1011
ProDye® Sanger Sequencing Standard
DNA of a known sequence template for control reactions prepared with ProDye® Terminator Sequencing System.
CR4604, CR4696
Frequently Used With
Spectrum Compact CE System
An affordable benchtop instrument for Sanger sequencing and fragment analysis.
CE1304, CE1307, CE1305
Spectrum Compact CE System Consumables
Plug-and-play prefilled consumables with guided software user interface for the Spectrum Compact CE System.
CE2300, CE2301, CE2302, CE2304, CE2404, CE2307, CE2407, CE2507, CE2308, CE2332, CE2340, CE2399
Wizard® MagneSil® Sequencing Reaction Clean-Up System
High-throughput purification of DNA from Sanger sequencing reactions.
A1831, A1832, A1835
