Rabbit Reticulocyte Lysate, Untreated
Characterize Protein Products, Study Transcriptional and Translational Control
- Contains tRNA, ribosomes, amino acids, initiation, elongation and termination factors needed for protein synthesis
- No micrococcal nuclease treatment
- Consistent reticulocyte production from every lot
Catalog Number:
Size
Catalog Number: L4151
Rabbit Reticulocyte Lysate, Untreated
Contains the cellular components necessary for protein synthesis (tRNA, ribosomes, amino acids, initiation, elongation and termination factors) but has not been treated with micrococcal nuclease. Untreated Lysate is used primarily for the isolation of these components and as an abundant source of endogenous globin mRNA. Untreated Lysate is prepared from New Zealand white rabbits in the same manner as treated lysates with the exception that it is not treated with micrococcal nuclease.
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- Isolation of the cellular components necessary for protein synthesis.
- Translation of exogenous mRNA, after further optimization, including the addition of energy-regenerating systems, hemin, endogenous tRNA and micrococcal nuclease to remove endogenous mRNA.
- Pelham, H.R.B. and Jackson, R.J. (1976) Eur. J. Biochem. 67, 247–56.
Protocols
Complete Protocol
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
|
Rabbit Reticulocyte Lysate, Untreated |
L415A | 1 × 1,000μl |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Resources
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