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SV Total RNA Isolation System

Z3101-sv-total-rna-isolation-system-trial-size Z3100-sv-total-rna-isolation-system-50-preps Z3105-sv-total-rna-isolation-system-250-preps

Extracts RNA from Tissues, Cultured Cells and White Blood Cells

  • Rapid isolation of high yields of total RNA without the use of hazardous compounds
  • Choice of microcentrifugation (spin) or vacuum purification methods
  • Purified RNA suitable for all routine molecular biology applications, including RT-PCR

Catalog Number:

Size

Catalog Number: Z3101

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Catalog Number: Z3100

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Catalog Number: Z3105

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Overview
Protocols
Specifications
Resources
Related Products

Isolate RNA with a Simple, 1-hour Procedure

The SV Total RNA Isolation System provides a fast and simple technique for preparation of intact total RNA from tissues, cultured cells and white blood cells in as little as one hour. Using this membrane-based purification system, up to 60mg of tissue can be processed per purification, depending on tissue type. The system incorporates a DNase treatment step directly on the minicolumn membrane. This step substantially reduces genomic DNA contamination, which can interfere with amplification-based methodologies. Purification is achieved without the use of phenol:chloroform extractions or ethanol precipitations, and there is no DNase carryover in the final RNA preparation

Sample Types Processed with the SV Total RNA Isolation System

Blood
Sample Type Promega External Comments
Human blood plasma and serum
Yes
Yes
A protocol is provided in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resource:
Fresh or once-frozen human serum (100μl) was added to 175μl of SV RNA Lysis Buffer, then the remainder of the SV Total RNA Isolation System protocol was followed.
Chen, X.Q. et al. (2000) Clin. Cancer Res. 6, 3823–6.
PubMed ID #11051224.
Human leukocytes
Yes
A protocol is provided in the SV Total RNA Isolation System Technical Manual #TM048.
Winter flounder red blood cells
Yes
Pedersen, S.F. et al. (2003) Am. J. Physiol. Cell Physiol. 284, C1561–76.
PubMed ID #12734109.
Human peripheral blood mononuclear cells
Yes
Yawalker, N. et al. (1998) J. Invest. Dermatol. 111, 1053–7.
PubMed ID #9856816.
Escoffier, C. et al. (1999) J. Virol. 73, 1695–8.
PubMed ID #9882382.
Primary CD4+ human T lymphocytes
Yes
Secchiero, P. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 14620–5.
PubMed ID #11114167.
Viral RNA from human plasma
Yes
Personal communication. Contact Promega Technical Services for more information.
Mammalian Tissues
Sample Type Promega External Comments
Mouse or rat brain
Yes
Yes
Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources:
Kephart, D. Measuring gene expression from mammalian brain tissue. eNotes
Brisco, P. Sankbeil, J. and Kephart, D. (1997) Promega Notes 64, 7–10
Kephart, D. and Hooper, K. (1998) Promega Notes 69, 17–9.
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
Mouse thoracic dorsal root ganglion neurons
Yes
Ekström, P.A.R. et al. (2003) Mol. Cell. Neurosci. 22, 383–95.
PubMed ID #12691739.
Mouse central nervous system
Yes
Serafini, B. et al. (2000) Am. J. Pathol. 157, 1991–2002.
PubMed ID #11106572.
Mouse small intestine
Yes
Serafini, B. et al. (2000) Am. J. Pathol. 157, 1991–2002.
PubMed ID #11106572.
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
Mouse muscle
Yes
Yes
Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources:
Sakai, H. et al. (1999) J. Physiol. 519, 323–33.
PubMed ID #10457052.
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
Mouse or rat liver
Yes
Yes
Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources:
Sakai, H. et al. (1999) J. Physiol. 519, 323–33.
PubMed ID #10457052.
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
Mouse kidney
Yes
Yes
Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources:
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
Attmane-Elakeb, A. et al. (2000) J. Biol. Chem. 275, 33548–53.
PubMed ID #10942780.
Mouse spleen
Yes
Yes
Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Serafini, B. et al. (2000) Am. J. Pathol. 157, 1991–2002.
PubMed ID #11106572.
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
Mouse embryonic heart, brain and skeletal muscle
Yes
Agarkova, I. et al. (2000) J. Biol. Chem. 275, 10256–64.
PubMed ID #10744711.
Mouse or rat lung
Yes
Yes
Agarkova, I. et al. (2000) J. Biol. Chem. 275, 10256–64.
PubMed ID #10744711.
Rat pancreas
Yes
Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources:
Brisco, P. Sankbeil, J. and Kephart, D. (1997) Promega Notes 64, 7–10.
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
Mouse or rat heart
Yes
Yes
Follow the standard protocol for tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources:
Brisco, P. Sankbeil, J. and Kephart, D. (1997) Promega Notes 64, 7–10.
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
Mouse colon
Yes
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
Mouse stomach
Yes
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
Mouse thymus
Yes
Pinto, D. et al. (1999) J. Biol. Chem. 274, 6476–82.
PubMed ID #10037740.
Mouse eyes
Yes
Lucas, R.J. et al. (1999) Science 284, 505–7.
PubMed ID #10205062.
Mouse retina
Yes
Freedman, M.S. et al. (1999) Science 284, 502–4.
PubMed ID #10205061.
Rat whole cochlea, modiola and cochlear sensorineural epithelial/lateral walls
Yes
Stöver, T. et al. (2000) Brain Res. Mol. Brain Res. 76, 25–35.
PubMed ID #10719212.
Human muscle
Yes
Roth, S.M. et al. (2002) Physiol. Genomics 10, 181–90.
PubMed ID #12209020.
Human placenta
Yes
Neuschäfer-Rube, F. et al. (2003) Biochem. J. 371, 443–9.
PubMed ID #12519077.
Gonzalez-Perrett, S. et al. (2001) Proc. Natl. Acad. Sci. USA 98, 1182–7.
PubMed ID #11252306.
Human skin
Yes
Yawalker, N. et al. (1999) J. Invest. Dermatol. 113, 43–8.
PubMed ID #10417617.
Yawalker, N. et al. (1998) J. Invest. Dermatol. 111, 1053–7.
PubMed ID #9856816.
Human brain
Yes
Miyasaka, T. et al. (2001) Am. J. Pathol. 158, 373–9.
PubMed ID #11159174.
Primary human peritoneal mesothelial cells
Yes
Cronauer, M.V. et al. (1999) Am. J. Pathol. 155, 1977–84.
PubMed ID #10595927.
Human cerebrovascular tissue
Yes
Cronauer, M.V. et al. (1999) Am. J. Pathol. 155, 1977–84.
PubMed ID #10595927.
Human adenoma tissue
Yes
Jaquet, P. et al. (2000) J. Clin. Endocrinol. Metab. 85, 781–92.
PubMed ID #10690891.
Human hair follicle
Yes
Use fresh samples and at least 10 or more hair roots. Align the hairs, and cut the root end from the hair and quickly process using the SV RNA Isolation System. The amount of RNA is very small and can generally only be detected by RT-PCR. Contact Promega Technical Services for more information.
Ferret aorta
Yes
Gangopadhyay, S.S. et al. (2002) Biochem. J. 372, 347–57.
PubMed ID #12603201.
Feline oral mucosa
Yes
Harley, R. et al. (1999) Clin. Diag. Lab. Immunol. 6, 471–8.
PubMed ID #10391845.
Plant Tissues
Sample Type Promega External Comments
Tomato leaf
Yes
Follow the standard protocol for plant tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources:
Shenoi, H. and Kephart, D. Titration of tomato leaf material used as starting material for SV total RNA isolation. eNotes
Kobs, G. (1998) Promega Notes 68, 28–31.
Arabidopsis thaliana leaf
Yes
Yes
Follow the standard protocol for plant tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources:
Technically Speaking: SV Total RNA Isolation System. Promega Notes 68, 16–9. Caldelari, D. et al. (2001) Plant Physiol. 126, 1416–29. PubMed ID #11500541.
Arabidopsis thaliana flower, stem and root
Yes
Caldelari, D. et al. (2001) Plant Physiol. 126, 1416–29.
PubMed ID #11500541.
Tobacco leaf
Yes
Follow the standard protocol for plant tissue in the SV Total RNA Isolation System Technical Manual #TM048.
Sugar beet 14-day seedling, cotyledon, hypocotyl, 46-day plant and 90-day plant)
Yes
Fowler, M.R. et al. (2000) J. Exp. Bot. 353, 2125–6.
PubMed ID #11141186.
Chicory taproot, etiolated heads, leaf, and green and etiolated seedlings
Yes
Fowler, M.R. et al. (2000) J. Exp. Bot. 353, 2125–6.
PubMed ID #11141186.
Three-day-old mung bean hypocotyls
Yes
Leiter, H. et al. (1999) J. Biol. Chem. 274, 21830–9.
PubMed ID #10419500.
Pea seedling leaf
Yes
Skaf, J.S. et al. (2000) J. Gen. Virol. 81, 1103–9.
PubMed ID #10725438.
Cultured Cells
Sample Type Promega External Comments
Primary rat endothelial cells
Yes
Thum, T., Haverich, A. and Borlak, J. (2000) FASEB J. 14, 740–51.
PubMed ID #10744630.
Fibroblasts derived from mouse embryonic stem cells
Yes
Zhang, S., Day, I.N.M. and Ye, S. (2001) Physiol. Genomics 5, 187–92.
PubMed ID #11328964.
Primary human coronary artery endothelial cells
Yes
Zhang, S., Day, I.N.M. and Ye, S. (2001) Physiol. Genomics 5, 187–92.
PubMed ID #11328964.
Bacteria
Sample Type Promega External Comments
Escherichia coli
Yes
Yes
Follow the standard protocol for bacteria in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources:
Kobs, G. (1998) Promega Notes 68, 28–31.
Ohmichi, T., Maki, A. and Kool, E.T. (2002) Proc. Natl. Acad. Sci. USA 99, 54–9.
PubMed ID #11752404.
Bacillus subtilis
Yes
Yes
Follow the standard protocol for bacteria in the SV Total RNA Isolation System Technical Manual #TM048.
Additional resources:
Kobs, G. (1998) Promega Notes 68, 28–31.
Colombo, M.L. et al. (2004) Antimicrob. Agents Chemother. 48, 484–90.
PubMed ID #14742199.
Vibrio vulnificus
Yes
Chen, Y.C. et al. (2004) Infect. Immun. 72, 629–36.
PubMed ID #14742502.
Salmonella enterica
Yes
Kelly, A. et al. (2004) Microbiology 150, 2037–53.
PubMed ID #15256548.
Klein, J.R., Fahlen, T.F. and Jones, B.D. (2000) Infect. Immun. 68, 3368–76.
PubMed ID #10816487.
Streptomyces coelicolor
Yes
The biomass from pregerminated Streptomycetes coelicolor spores was collected, frozen in liquid nitrogen and crushed by mortar and pestle. The resulting powder was processed to extract RNA using the TRIzol® reagent, and the aqueous phase was treated with DNase I. SV RNA Dilution Buffer (375µl) was added to the nucleic acid solution, and samples were mixed and centrifuged. The resulting supernatant was mixed with 250µl of cold 96% ethanol, loaded on an SV spin column and centrifuged for 1 minute. The column was then processed as described in the SV Total RNA Isolation System Technical Manual #TM048.
Van Keulen G. et al. (2004) J. Microbiol. Methods 58, 139–42.
PubMed ID #15177913.
Bacillus thuringiensis
Yes
Lee, M.K. et al. (2003) Appl. Environ. Microbiol. 69, 4648–57.
PubMed ID #12902253.
Alteromonas
Yes
Orikoshi, H. et al. (2003) J. Bacteriol. 185, 1153–60.
PubMed ID #12562783.
Tsujibo, H. et al. (2000) Appl. Environ. Microbiol. 66, 3778–83.
PubMed ID #10966390.
Pseudomonas aeruginosa
Yes
After resuspension in a lysis buffer (50mM Tris-HCl [pH 7.5], 150mM NaCl), the cell pellet was subjected to French press, and 100μl aliquots were processed with the SV Total RNA Isolation System as directed by the manufacturer. Cambronne, E.D. and Schneewind, O. (2002) J. Bacteriol. 184, 5880–93.
PubMed ID #12374821.
Streptococcus pneumoniae
Yes
Brown, J.S. et al. (2002) Infect. Immun. 70, 4389–98.
PubMed ID #12117949.
Mycobacterium smegmatis
Yes
Milano, A. et al. (2001) J. Bacteriol. 183, 6801–6.
PubMed ID #11698368.
Klebsiella pneumoniae
Yes
Yigit, H. et al. (2001) Antimicrob. Agents Chemother. 45, 1151–61.
PubMed ID #11257029.
Rhizobium leguminosarum
Yes
Lee, H.Y., An, J.H. and Kim, Y.S. (2000) Eur. J. Biochem. 267, 7224–9.
PubMed ID #111064359.
Pseudomonas putida
Yes
Cowles, C.E., Nichols, N.N. and Harwood, C.S. (2000) J. Bacteriol. 182, 6339–46.
PubMed ID #11053377.
Grimm, A.C. and Harwood, C.S. (1999) J. Bacteriol. 181, 3310–6.
PubMed ID #10322041.
Rhodopseudomonas palustris
Yes
Pelletier, D.A. and Harwood, C.S. (2000) J. Bacteriol. 182, 2753–60.
PubMed ID #107815431.
Neiserria meningitidis
Yes
Deghmane, A.E. et al. (2000) EMBO J. 19, 1068–78.
PubMed ID #106989471.
Lactobacillus helveticus
Yes
Pederson, J.A. et al. (1999) J. Bacteriol. 181, 4592–7.
PubMed ID #10419958.
Simkania negevensis ZT
Yes
Everett, K.D. et al. (1999) J. Bacteriol. 181, 4734–40.
PubMed ID #10438738.
Chlamydia trachomatis
Yes
Everett, K.D. et al. (1999) J. Bacteriol. 181, 4734–40.
PubMed ID #10438738.
Other Organisms and Tissues
Sample Type Promega External Comments
Gigaspora margarita
Yes
Lanfranco, L. et al. (2002) Plant Physiol. 130, 58–67.
PubMed ID #12226486.
Trichomonas vaginalis
Yes
Coombs, G.H. et al. (2004) J. Biol. Chem. 279, 5249–56.
PubMed ID #14630923.
Pacific oyster larvae
Yes
Steele, S. and Thorndyke, M. (1999) Promega Notes 71, 2–4.
Cartilaginous fish brain
Yes
Pederson, J.A. et al. (1999) J. Bacteriol. 181, 4592–7.
PubMed ID #10419958.
Zebrafish gut
Yes
Crosnier, C. et al. (2005) Development 132, 1093–104.
PubMed ID #15689380.
Drosophila larva tissue
Yes
Petryk, A. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 13773–8.
PubMed ID #14610274.
Frog liver
Yes
Saeed-Kothe, A. and Powers-Lee, S.G. (2003) J. Biol. Chem. 278, 26722–6.
PubMed ID #12738780.
Frog gonads and mesonephroi
Yes
Ogata, M. et al. (2003) Genetics, 164, 613–20.
PubMed ID #12807781.
Chicken embryonic heart, brain and skeletal muscle
Yes
Agarkova, I. et al. (2000) J. Biol. Chem. 275, 10256–64.
PubMed ID #10744711.
Venom sacs and glands from Naja Atra (Asian cobra)
Yes
Lou, X. et al. (2004) J. Biol. Chem. 279, 39094–104.
PubMed ID #15252034.
Microcystis aeruginosa
Yes
Kaebernick, M. et al. (2002) Appl. Environ. Microbiol. 68, 449–55.
PubMed ID #11823177.
Miscellaneous
Sample Type Promega External Comments
Human immunodeficiency virus
Yes
Personal communication. Contact Promega Technical Services for more information.
RNA from soil
Yes
Personal communication. Contact Promega Technical Services for more information.

Specifications

Catalog Number:

What's in the box

Item Part # Choose a size Available Separately

Collection Tubes

 A130E 2 × 5/pk

Nuclease-Free Water

 P119A 1 × 1,250μl View Product

Elution Tubes

 Z200A 2 × 5/pk

RNA Lysis Buffer (RLA)

 Z305B 1 × 10ml View Product

RNA Dilution Buffer (RDA)

 Z306B 1 × 4ml

RNA Wash Solution (RWA)

 Z309B 1 × 11.8ml View Product

DNase Stop Solution (DSA)

 Z312A 1 × 5.3ml

Yellow Core Buffer

 Z317A 1 × 2.5ml

MnCl2, 0.09M

 Z318A 1 × 250μl

DNase I (lyophilized)

 Z358B 1 × 1 vial View Product

Spin Columns

 Z503B 2 × 5/pk

Beta-Mercaptoethanol

 Z559A 1 × 900μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

What's in the box

Item Part # Choose a size Available Separately

Collection Tubes

 A130D 2 × 25/pk

Nuclease-Free Water

 P119E 1 × 13ml View Product

Elution Tubes

 Z200B 2 × 25/pk

RNA Lysis Buffer (RLA)

 Z3051 1 × 50ml View Product

RNA Dilution Buffer (RDA)

 Z306A 1 × 20ml

RNA Wash Solution (RWA)

 Z3091 1 × 58.8ml View Product

DNase Stop Solution (DSA)

 Z312A 1 × 5.3ml

Yellow Core Buffer

 Z317A 1 × 2.5ml

MnCl2, 0.09M

 Z318A 1 × 250μl

DNase I (lyophilized)

 Z358A 1 × 1 vial View Product

Spin Columns

 Z503C 2 × 25/pk

Beta-Mercaptoethanol

 Z559A 1 × 900μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

What's in the box

Item Part # Choose a size Available Separately

Collection Tubes

 A130A 5 × 50/pk

Nuclease-Free Water

 P119C 2 × 25ml View Product

Elution Tubes

 Z200C 5 × 50/pk

RNA Lysis Buffer (RLA)

 Z305E 1 × 250ml View Product

RNA Dilution Buffer (RDA)

 Z306C 1 × 96ml

RNA Wash Solution (RWA)

 Z309D 1 × 206ml View Product

DNase Stop Solution

 Z312D 1 × 26.5ml

Yellow Core Buffer

 Z317C 1 × 11ml

MnCl2, 0.09M

 Z318B 2 × 750μl

DNase I (lyophilized)

 Z358A 5 × 1 vial View Product

Spin Columns

 Z503D 5 × 50/pk

Beta-Mercaptoethanol

 Z559A 1 × 900μl

SDS

Search for SDS

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

DD

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