MagneSil® Genomic, Fixed-Tissue System
Magnetic Method to Purify DNA from FFPE Tissue
- One-hour purification after overnight proteinase K digestion
- No centrifugation of lysate required
- Amplifiable DNA from 10µM FFPE tissue slices
Catalog Number:
Size
Catalog Number: MD1490
Amplification-Ready DNA from FFPE Tissue in 1 Hour
The MagneSil® Genomic, Fixed Tissue System provides a fast, simple technique for extracting genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissue. After an overnight Proteinase K digestion, genomic DNA can be manually purified from FFPE thin tissue sections in less than an hour, and up to 12 samples can be processed at a time using the MagneSphere® Technology Magnetic Separation Stand (twelve-position) (Cat.# Z5342).
The optimized protocol and wash buffers yield genomic DNA that is largely free of small DNAs, which are potent inhibitors of PCR amplification. Amplifiable genomic DNA can be isolated from 10μm thin sections without centrifugation of the lysate prior to purification. Targets as large as 450–1,800bp can be amplified, and multiplex PCR can be performed using DNA purified with this system.
Protocols
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size | Concentration | Available Separately |
|---|---|---|---|---|
|
Resin |
A825C | 1 × 900μl | View Product | |
|
Lysis Buffer |
A826C | 1 × 40ml | View Product | |
|
2X Wash Buffer |
A827C | 1 × 30ml | View Product | |
|
Elution Buffer |
A828C | 1 × 15ml | View Product | |
|
Incubation Buffer |
D920C | 1 × 35ml | ||
|
DTT |
V125A | 1 × 1.125ml | 1M | |
|
Proteinase K |
V302A | 2 × 10mg | View Product |
SDS
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Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
Resources
Inne
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