Trypsin/Lys-C Mix, Mass Spec Grade
Improve Protein Digestion with Mix of Trypsin Gold and rLys-C
- Standard overnight digestion, nondenaturing conditions
- Increased peptide recovery, enhanced protein quantitation, improved reproducibility
- Tolerant to trypsin-inhibiting contaminants
Catalog Number:
Size
Catalog Number: V5071
Catalog Number: V5072
Catalog Number: V5073
Trypsin/Lys-C Mix, Mass Spec Grade: See What You've Been Missing
Using standard digestion conditions, Trypsin/Lys-C Mix improves peptide digestion over trypsin alone, increasing peptide recovery and resulting in better protein quantitation.
Increased Peptide and Protein Identifications Using a Standard Trypsin Digestion Protocol
Improved Reproducibility using Trypsin/Lys-C
Tolerant To Trypsin-Inhibiting Contaminants
Efficient digestion with Trypsin/Lys-C Mix in the presence of trypsin-inhibiting agents. Yeast protein extract was used as a model system.
Conclusions: Use of Trypsin/Lys-C resulted in fewer missed cleavages and a 20% increase in protein identifications.
Conclusions: Use of Trypsin/Lys-C resulted in fewer missed cleavages and a 20% increase in protein identifications.
Protocols
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
|
Resuspension Buffer |
V181A | 1 × 500μl |
Trypsin/Lys-C Mix, Mass Spec Grade |
V507A | 1 × 20μg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
|
Resuspension Buffer |
V181A | 1 × 500μl |
Trypsin/Lys-C Mix, Mass Spec Grade |
V507B | 1 × 100μg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
|
Resuspension Buffer |
V181A | 1 × 500μl |
Trypsin/Lys-C Mix, Mass Spec Grade |
V507A | 5 × 20μg |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
Related Products
Similar Products
6 × 5 LC-MS/MS Peptide Reference Mix
Peptide mix and free software to monitor LC/MS instrument performance.
V7491, V7495
Rapid-Digestion Trypsin and Trypsin/Lys-C Kits
Streamlined protocol for fast, efficient protein digestion.
VA1060, VA1061
rAsp-N, Mass Spec Grade
Cleavage site: N-terminal of Asp. Optimal pH: 6–9.
VA1160
Frequently Used With
Magnetic Proteomics Sample Prep (MPSP) Kit
Magnetic beads for streamlined, SP3-based proteomics sample preparation.
CS3325A04
Arg-C, Sequencing Grade
Cleavage site: C-terminal of Arg. Optimal pH: 7.6–7.9.
V1881
Asp-N, Sequencing Grade
Cleavage site: N-terminal of Asp. Optimal pH: 4–9.
V1621
Chymotrypsin, Sequencing Grade
Cleavage sites: C-terminal of Tyr, Phe, Trp. Optimal pH: 7–9.
V1061, V1062
