Plexor® HY System
qPCR Assay to Determine Male and Total Human DNA Concentrations
- Simultaneously quantify autosomal and Y-chromosome DNA
- Routinely detect down to 6.4pg of DNA
- Internal positive control/melt-curve analysis guard against false-negative and false-positive results
Catalog Number:
Choose a product
Size
Catalog Number: DC1001
Catalog Number: DC1000
Catalog Number: DC1500
The Power to See It All in a Single Assay
The Plexor® HY System is a real-time PCR assay to determine the concentration of total human DNA and male human DNA simultaneously in one reaction. The kit contains an internal PCR control (IPC) to test for false-negative results that may occur in the presence of PCR inhibitors and a melt-curve function to confirm that the correct product was amplified. This sensitive multiplex kit provides consistent DNA detection down to 6.4pg of total DNA. PCR setup is performed at room temperature and is compatible with automated platforms.
The Plexor® HY System is compatible with automated platforms and optimized for use on the Applied Biosystems 7500 and 7500 FAST real-time PCR systems and Stratagene Mx3005P® and Mx3000P® qPCR systems. For information about use with other qPCR instrumentation, contact Technical Services.
The Plexor® Analysis Software is available for free download. The unique functions of this software allow you to quickly and easily review data and create reports. Replicate samples are automatically averaged, template amounts are calculated and the necessary volume of DNA is displayed for your optimized STR amplification conditions.
Promega forensic products are manufactured in alignment with the ISO 18385 standard. This standard ensures minimal risk of human DNA contamination for products used to collect, store and analyze biological materials for forensic purposes. Learn more.
If There is DNA to Detect, Plexor® HY System Will Show It
The system works by measuring a reduction in fluorescent signal during amplification. Amplification of each target uses only two primers, one of which contains both a fluorescent tag and a modified base. As amplification proceeds, fluorescence is reduced by site-specific incorporation of a fluorescent quencher opposite the complementary modified base. The quencher is in close proximity to a fluorescent dye located on the end of the primer, resulting in a reduction of fluorescent signal. After PCR, a melt-curve analysis can be performed to provide an internal control for the final assay design or to expedite troubleshooting.
Want to Automate?
Save time and improve precision by automating your DNA quantification setup. The PowerQuant® and Plexor® HY Systems can be automated on the Biomek® 4000 (Beckman Coulter), Freedom EVO (Tecan), or MicroLab STAR (Hamilton) liquid handlers. Additionally, import the quantification data into the STR Normalization Manager Software to automate your PCR amplification setup.
Automate your DNA Quantification Setup
Contact your local representative to receive a quote for the required quantities and to purchase and schedule the required installation and training services.
Protocols
Complete Protocol
Quick Protocols
Specifications
Catalog Number:
What's in the box
| Item | Part # | Choose a size | Concentration | Available Separately |
|---|---|---|---|---|
|
Plexor® HY Male Genomic DNA Standard |
DD149A | 1 × 150μl | 50ng/μl | |
|
Plexor® HY 20X Primer/IPC Mix |
DK716A | 1 × 200μl | ||
|
Plexor® HY 2X Master Mix |
DP120A | 2 × 1ml | ||
|
Water, Amplification Grade |
DW099A | 2 × 1,250μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
CAL Fluor® technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc., for research and development and forensic and paternity testing. These products are sold for use by the end-user only and may not be resold, distributed or repackaged.
The purchase of this product conveys to the buyer the limited, nonexclusive, nontransferable right (without the right to resell, repackage, or further sublicense) under U.S. Pat. Nos. 7,422,850, 7,517,651 and 7,541,147 to use the product. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include or carry any right or license to sell this product. For information on purchasing a license for other uses, please contact Promega Corporation, Business Development, 2800 Woods Hollow Road, Madison, WI 53711, or EraGen Biosciences, Corporate Licensing, 918 Deming Way, Suite 201, Madison, WI 53717. Phone (608) 662-9000; Fax (608) 662-9003.
What's in the box
| Item | Part # | Choose a size | Concentration | Available Separately |
|---|---|---|---|---|
|
Plexor® HY Male Genomic DNA Standard |
DD149A | 3 × 150μl | 50ng/μl | |
|
Plexor® HY 20X Primer/IPC Mix |
DK716A | 4 × 200μl | ||
|
Plexor® HY 2X Master Mix |
DP120A | 8 × 1ml | ||
|
Water, Amplification Grade |
DW099A | 5 × 1,250μl | View Product |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
CAL Fluor® technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc., for research and development and forensic and paternity testing. These products are sold for use by the end-user only and may not be resold, distributed or repackaged.
The purchase of this product conveys to the buyer the limited, nonexclusive, nontransferable right (without the right to resell, repackage, or further sublicense) under U.S. Pat. Nos. 7,422,850, 7,517,651 and 7,541,147 to use the product. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include or carry any right or license to sell this product. For information on purchasing a license for other uses, please contact Promega Corporation, Business Development, 2800 Woods Hollow Road, Madison, WI 53711, or EraGen Biosciences, Corporate Licensing, 918 Deming Way, Suite 201, Madison, WI 53717. Phone (608) 662-9000; Fax (608) 662-9003.
What's in the box
| Item | Part # | Choose a size |
|---|---|---|
|
Plexor® Concentrated Fluorescein Calibrator |
DS101A | 1 × 50μl |
|
Plexor® Concentrated CAL Fluor® Orange 560 Calibrator |
DS102A | 1 × 50μl |
|
Plexor® Concentrated CAL Fluor® Red 610 Calibrator |
DS103A | 1 × 50μl |
|
Plexor® Concentrated IC5 Calibrator |
DS104A | 1 × 50μl |
|
Plexor® 1X Calibration Buffer |
DS105A | 1 × 11ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
Not For Medical Diagnostic Use.Storage Conditions
CAL Fluor® technology is the subject of pending patents and is licensed and sold under agreement with Biosearch Technologies, Inc., for research and development and forensic and paternity testing. These products are sold for use by the end-user only and may not be resold, distributed or repackaged.
Resources
Software and Firmware
Related Products
Similar Products
Frequently Used With
PowerPlex® Fusion System
Co-amplification and fluorescent detection of 24 loci.
DC2402, DC2408
PowerPlex® Y23 System
5-dye STR multiplex for amplification of 23 Y-STR loci.
DC2305, DC2320, DM6571
PowerPlex® ESX 16 and ESI 16 Fast Systems
Co-amplification and 5-color detection of ENFSI-recommended STR loci.
DC1610, DC1611, DC1630, DC1631, DC1620, DC1621
PowerPlex® ESX 17 and ESI 17 Fast Systems
Co-amplification and 5-color detection of ENFSI-recommended STR loci.
DC1710, DC1711, DC1720, DC1721, DC1731, DC1730
