DeadEnd™ Colorimetric TUNEL System
A Modified TUNEL Assay for Detection of Apoptotic Cells In Situ
- Detects apoptosis in tissue sections or cell cultures
Catalog Number:
Size
Catalog Number: G7360
Catalog Number: G7130
The DeadEnd™ Colorimetric TUNEL System provides the reagents to perform terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) of fragmented nuclear DNA to assess apoptosis in situ at the single-cell level in tissue sections or cultured cells. Biotinylated nucleotide is incorporated at the 3´-OH DNA ends of the fragmented DNA using Terminal Deoxynucleotidyl Transferase (TdT). Horseradish-peroxidase-labeled streptavidin (Streptavidin HRP) is then bound to these biotinylated nucleotides, which are detected using the stable chromogen, diaminobenzidine (DAB). Using this procedure, apoptotic nuclei are stained dark brown and visualized by light microscopy.
Features and Benefits
- Detects apoptosis in thick tissue sections or assess cell morphology.
- Assay includes DAB reagent for color detection and plastic coverslips that simplify sample handling.
- Proven applications include Vibratome® sections of neuronal tissue, Jurkat cells, HL-60 cells.
Note: The protocol for the DeadEnd™ TUNEL Assay recommends an optional DNase I treatment of samples as a positive control to detect DNA fragmentation. RQ1 RNase-Free DNase (Cat.# M6101) can be used to generate the positive control and is available separately.
Protocols
Specifications
Catalog Number:
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Plastic Coverslips |
G326B | 1 × 20 each | |
|
Equilibration Buffer |
G327B | 1 × 4.8ml | |
|
SSC, 20X |
G329B | 1 × 20ml | |
|
Streptavidin HRP |
G714A | 1 × 40μl | |
|
Biotinylated Nucleotide Mix |
G715A | 1 × 20μl | |
|
Terminal Deoxynucleotidyl Transferase (recombinant) |
M828B | 1 × 600u | |
|
Proteinase K |
V302A | 1 × 10mg | View Product |
DAB 10X Chromogen |
J293A | 1 × 400μl | |
DAB Substrate 1X Buffer |
J294A | 1 × 3.6ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
What's in the box?
| Item | Part # | Size | Available Separately |
|---|---|---|---|
|
Plastic Coverslips |
G326B | 2 × 20 each | |
|
Equilibration Buffer |
G327C | 1 × 9.6ml | |
|
SSC, 20X |
G329A | 1 × 70ml | |
|
Streptavidin HRP |
G714A | 1 × 40μl | |
|
Biotinylated Nucleotide Mix |
G715A | 2 × 20μl | |
|
Terminal Deoxynucleotidyl Transferase (recombinant) |
M828B | 2 × 600u | |
|
Proteinase K |
V302A | 1 × 10mg | View Product |
DAB 10X Chromogen |
J293A | 1 × 400μl | |
DAB Substrate 1X Buffer |
J294A | 1 × 3.6ml |
SDS
Search for SDSCertificate of Analysis
Use Restrictions
For Research Use Only. Not for Use in Diagnostic Procedures.Storage Conditions
See Protocol for detailed storage recommendations.
Resources
Other Resources
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RQ1 RNase-Free DNase
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M6101
