| Cell Line |
Transfection Reagent |
Type of Nucleic Acid Transfected |
Plate Format |
Amount of Nucleic Acid Used (µg) |
Ratio of Reagent to Nucleic Acid (µl:µg) |
Journal Citation |
HEK 293 |
FuGENE HD Transfection Reagent |
DNA |
100mm dish |
9 |
3:1 |
2013. J. Cell Biol. 202: 579–95 |
Description of cell line: adenovirus-transformed human embryonic kidney
Additional Relevant Information: 5x10e6 cells seeded per plate, study used HEK293-AAV cells
Type of Transfection:
|
 |
HEK 293 |
FuGENE HD Transfection Reagent |
DNA |
24-well plate |
0.3 |
not reported |
2010. J. Biol. Chem. 285(41): 31313-24 |
Description of cell line: adenovirus-transformed human embryonic kidney
Additional Relevant Information:
Type of Transfection:
|
|
HEK 293 |
FuGENE HD Transfection Reagent |
DNA |
6-well plate |
not reported |
not reported |
2012. Development 139: 3986-96 |
Description of cell line: adenovirus-transformed human embryonic kidney
Additional Relevant Information: plated 1x10e6 cells/well
Type of Transfection:
|
|
HEK 293 |
FuGENE HD Transfection Reagent |
DNA |
96-well plate |
0.1 |
3:1 |
2012. J. Biol. Chem. 287: 36370–83 |
Description of cell line: adenovirus-transformed human embryonic kidney
Additional Relevant Information: 2x10e4 cells plated per well
Type of Transfection:
|
|
HEK 293T |
FuGENE 6 Transfection Reagent |
DNA |
100 mm dish |
4 |
10:4 |
1999. J. Cell Biol. 147: 77–88 |
Description of cell line: adenovirus-transformed human kidney cells, expressing SV40 large T antigen
Additional Relevant Information: growth medium without serum; 25% confluent
Type of Transfection:
|
|
HEK 293T |
FuGENE 6 Transfection Reagent |
DNA |
100 mm dish |
5 |
2.5:1 |
2011. J. Biol. Chem. 286: 31092–31104 |
Description of cell line: adenovirus-transformed human kidney cells, expressing SV40 large T antigen
Additional Relevant Information: 5x10e6 cells/dish
Type of Transfection:
|
|
HEK 293T |
FuGENE 6 Transfection Reagent |
DNA |
12-well plate |
0.2 |
2.5:1 |
2011. J. Biol. Chem. 286: 31092–31104 |
Description of cell line: adenovirus-transformed human kidney cells, expressing SV40 large T antigen
Additional Relevant Information: 3x10e5 cells/well
Type of Transfection:
|
|
HEK 293T |
FuGENE 6 Transfection Reagent |
DNA |
24-well plate |
0.5 |
not reported |
1999. J. Biol. Chem. 274: 32091–32098 |
Description of cell line: adenovirus-transformed human kidney cells, expressing SV40 large T antigen
Additional Relevant Information: 5x10e4 cells plated
Type of Transfection:
|
|
HEK 293T |
FuGENE 6 Transfection Reagent |
DNA |
35 mm dish |
4 |
not reported |
1999. J. Biol. Chem. 274: 30778–30783 |
Description of cell line: adenovirus-transformed human kidney cells, expressing SV40 large T antigen
Additional Relevant Information: 2x10e5 cells plated
Type of Transfection:
|
|
HeLa |
FuGENE 6 Transfection Reagent |
DNA |
24-well plate |
0.5 |
not reported |
2000. J. Biol. Chem. 275: 12298-305 |
Description of cell line: human cervical cancer
Additional Relevant Information: cells plated at 6x10e4 cells/well 1day prior to transfection
Type of Transfection:
|
|
HeLa |
FuGENE 6 Transfection Reagent |
DNA |
35 mm dish |
4 |
3:2 |
|
Description of cell line: human cervical cancer
Additional Relevant Information:
Type of Transfection:
|
|
HeLa |
FuGENE 6 Transfection Reagent |
DNA |
6-well plate |
1 |
3:1 |
2003. J. Biol. Chem. 278: 6787-6794 |
Description of cell line: human cervical cancer
Additional Relevant Information: Cells plated at 2.5x10e4/well
Type of Transfection:
|
|
HeLa |
FuGENE 6 Transfection Reagent |
DNA |
96-well plate |
not reported |
3:1 |
|
Description of cell line: human cervical cancer
Additional Relevant Information: 2,500-5000 cells plated
Type of Transfection:
|
|
HeLa |
FuGENE HD Transfection Reagent |
DNA |
12-well plate |
0.5 |
3:1 |
2012. FASEB J. 26: 1629-39 |
Description of cell line: human cervical cancer
Additional Relevant Information: cells seeded at 100,000 cells/well one day prior to transfection
Type of Transfection:
|
|
HeLa |
FuGENE HD Transfection Reagent |
DNA |
24-well plate |
0.5 |
2:1 |
2013. mBio epub: e00418–12 |
Description of cell line: human cervical cancer
Additional Relevant Information: 5x10e4 cells seeded on coverslips in 24well plates for confocal microscopy
Type of Transfection:
|
|
HeLa |
FuGENE HD Transfection Reagent |
DNA |
24-well plate |
0.5 |
3.5:1 |
2011. Mol. Cell. Biol. 31: 935–54 |
Description of cell line: human cervical cancer
Additional Relevant Information: 0.9X10e5 cells/well plated 16hr before transfection
Type of Transfection:
|
|
HeLa |
FuGENE HD Transfection Reagent |
DNA |
24-well plate |
1 |
3:1 |
2013. J. Virol. 87: 2109–19 |
Description of cell line: human cervical cancer
Additional Relevant Information: 5x10e4 cells plated per well
Type of Transfection:
|
|
HeLa |
FuGENE HD Transfection Reagent |
DNA |
6-well plate |
2.5 |
8:2.5 |
2013. J. Bacteriol. 195: 708–16 |
Description of cell line: human cervical cancer
Additional Relevant Information: 2x10e5 cells seeded onto coverslips 1 day prior to transfection
Type of Transfection:
|
|
HeLa |
FuGENE HD Transfection Reagent |
DNA |
12-well plate |
0.2 |
not reported |
2012. Mol. Biol. Cell 23: 3499-510 |
Description of cell line: human cervical cancer
Additional Relevant Information: cells plated on glass coverslips at 1-1.3x10e5 cells/well one day prior to transfection, HeLa Tet-On cells were used
Type of Transfection:
|
|
Hep-2 |
FuGENE HD Transfection Reagent |
DNA |
12-well plate |
1 |
3:1 |
2013. J. Virol. 87: 2109–19 |
Description of cell line: HeLa derivative
Additional Relevant Information: 5x10e4 cells per well on glass coverslips
Type of Transfection:
|
|