IAC RT-qPCR Inhibition Control Assay, CAL Fluor® 560 and IPC qPCR Inhibition Control Assay, CAL Fluor® 560 Technical Manual
Instructions for Use of Product(s)
AM2030, AM2040
Literature # TM657
The use of an exogenous internal control in PCR provides full confidence in qPCR and RT-qPCR results and data interpretation. PCR or RT-PCR inhibitors, pipetting errors and thermocycler malfunctions are common causes of inconsistent results, and can be easily controlled for by including an Internal Positive Control (IPC) in the hydrolysis probe-based multiplexed amplification reaction. IPC provides information on DNA polymerase performance in PCR. IAC stands for Internal Amplification Control and provides information on reverse transcriptase and DNA polymerase performance in a one-step RT-qPCR reaction.
The IAC RT-qPCR and IPC qPCR Inhibition Control Assays can be used with any DNA polymerase, including those found in GoTaq®, GoTaq® Enviro RT-qPCR and GoTaq® Enviro qPCR Systems.
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