pGL4.72[hRlucCP] Vector Protocol
Instructions for Use of Product(s)
Literature # 9PIE690
The pGL4.72[hRlucCP] Vector encodes the luciferase reporter gene hRlucCP (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression.
The pGL4.72[hRlucCP] Vector has no promoter. However, it contains a multiple cloning region that allows for cloning of a promoter of choice. The hRlucCP reporter gene contains two protein destabilization sequences, hCL1 and hPEST. The protein encoded by hRlucCP responds more quickly and with greater magnitude to changes in transcriptional activity than the hRluc gene, its more stable counterpart.
Printed in USA. Revised 9/16