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Notes: cDNA fragments from the Nogo gene were amplified from genomic DNA and cloned into the pGEM^®-T vector. The Wizard^® Plus Mini- and Midiprep kits were used to purify the plasmids from bacterial cells. (2658)

Notes: Plasmids were purified with either the Wizard^® Plus Midipreps DNA Purification System or the Wizard^® Plus Megapreps DNA Purification System. The purified plasmids were used for in vitro transcription. (1209)

Notes: Plasmids were purified with either the Wizard^® Plus Minipreps DNA Purification System or the Wizard^® Plus Midipreps DNA Purifications System. PCR products were purified with the Wizard^® PCR Preps System. (0751)

Notes: The authors used the PolyATtract^® mRNA Isolation System to isolate mRNA from total RNA. They used this mRNA-rich fraction for primer extension analysis using Promega's AMV Reverse Transcriptase. The Wizard^® Plus Midiprep DNA Purification System was used for various plasmid isolations and the Erase-a-Base^®  System was used to generate deletion series. The Dual-Luciferase^® Reporter Assay System was used to study promoters cloned into the pGL3-Basic Vector. The pRL-SV40 was used as a transfection control plasmid. (0095)

Notes: DNA was isolated with the Wizard^® Plus DNA Purification System and ethanol-precipitated with ammonium acetate prior to transfection. The DNA was used for calcium phosphate transfection of PC12 and HepG2 cells. (0283)