Recombinant PNGase F for the Analysis of Glycoproteins Poster

Abstract

PNGase F is an endoglycosidase isolated from Elizabethkingia miricola that specifically removes N-linked glycans from glycoproteins. PNGase F is a commonly used reagent in workflows for identifying sites of N-linked glycosylation and characterizing N-linked glycan structures on therapeutic proteins.

We have expressed and purified recombinant PNGase F (rPNGase F) to homogeneity and shown that it is highly active against a wide panel of substrates including purified glycoproteins and complex mixtures of human serum glycoproteins. Furthermore, the enzyme is active in MS-compatible workflows both in non-denaturing conditions and in the presence of ProteaseMAX surfactant.