Cytochrome P450 Activity Screening in Low-Volume 384- and 1536-Well Plate Formats...

Abstract

Tracy Worzella, M.S.¹, Brad Larson, B.A.¹, Michael Bjerke, M.S.¹, Aoife Gallagher, Ph.D.², and Eric Matthews, M.S.^3
1Promega Corporation, Madison, WI, ²Deerac Fluidics, Dublin, Ireland, ³BMG LABTECH, Offenburg, Germany

Here we demonstrate the scalability of in vitro luminescent P450-Glo™ Assays for high-throughput cytochrome P450 activity screening. Deerac Fluidics’ Equator™ NS 808 Eight Tip Low Volume Dispenser was used for non-contact delivery of sample and reagent. The multifunctional, PMT-based BMG LABTECH PHERAstar reader was used to gather data. Z’-factor and inhibitor screen data in low-volume 384 and 1536-well formats demonstrate the scalability and functionality of these assays in a miniaturized format, making them ideal for high-throughput screening applications.