Promega Corporation

A Rapid and Sensitive Mix and Measure Luminescent Cell Viability Assay Scientific Poster

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Abstract

Richard A. Moravec, Michael T. Beck, Nancy Murphy, Mary Sobol, Rita R. Hannah, Keith V. Wood and Terry L. Riss
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711

We have developed a simple and highly sensitive homogeneous assay (CellTiter-Glo™ Assay) for quantifying viable cells in multi-well plates. The assay is based on detection of adenosine triphosphate (ATP), which is an indicator of viability present in all cells. The homogeneous “add-mix-measure” protocol is performed by adding a single reagent directly to cells cultured in serum-containing medium, mixing, and recording luminescence with a luminometer or CCD imaging device. The luminescence is proportional to ATP concentration and correlates to the number of viable cells over 3-4 logs depending on the type of multiwell plates and cells used. Assay sensitivity is sufficient to detect 4 cells/well in a 384 well format. The bioluminescent “glow” signal can be read within 10 minutes of reagent addition and has a half-life of ~5 hours, allowing flexibility for batch or continuous automated processing. We will demonstrate the usefulness of the CellTiter-Glo™ Assay for high-throughput cytotoxicity

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  • Part# PS012
  • Printed in USA.

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