Promega Corporation

The MagZ™ System: His-Tagged Protein Purification without Hemoglobin Contamination

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Abstract

This novel protein purification system opens up the possibilities for the expression, purification and functional analysis of His-tagged proteins expressed in rabbit reticulocyte-based protein expression systems. The MagZ™ Protein Purification System offers a unique advantage over nickel-based systems by specifically purifying polyhistidine-tagged (His-tagged) proteins from rabbit reticulocyte lysate without co-purification of hemoglobin. Thus, the proteins purified using the MagZ™ System could be used for several downstream applications, which are often limited by the presence of hemoglobin in the eluted samples using other immobilized metal ion affinity chromatography (IMAC) methods.

Promega Notes 88, 9–12.

Becky Godat, Laurie Engel and Tonny Johnson

Promega Corporation
Publication Date: 2004

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