Promega Corporation

In Vitro HisTag Pull-Down Assay Using MagZ™ Particles

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Abstract

Recently we developed a novel product, the MagZ™ Protein Purification System, for the purification of expressed his-tagged protein from rabbit reticulocyte lysate. This protein purification system has a unique advantage over nickel-based immobilized metal ion affinity chromatography (IMAC) systems in that it specifically purifies His-tagged proteins from rabbit reticulocyte lysate without co-purifying hemoglobin. This article demonstrates that MagZ™ Binding Particles can be used to develop in vitro His-tag pull-down assays. By using a His-tagged Id protein attached to MagZ™ Particles, we show that we can detect the interaction with MyoD.

Promega Notes 88, 13–15.

Becky Godat and Tonny Johnson

Promega Corporation
Publication Date: 2004

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