Promega Corporation

High-Throughput Purification Using the SV 96 Total RNA Isolation System

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Abstract

This article introduces the SV 96 RNA Isolation System. The system is used to purify high-quality total RNA with a flexible method that can be performed manually or automated on a liquid handling workstation such as the Beckman Biomek® 2000 or the Biomek® FX. The unique design of the SV 96 Total RNA Isolation System eliminates waste handling, includes a DNase treatment step to eliminate genomic DNA and allows consistent and reliable recovery of total RNA from tissue culture cells. The purified RNA is functionally tested in RT-PCR and results are shown to be consistent with those obtained using other RNA purification methods.

Promega Notes 79, 29–32.

Terri Grunst

Promega Corporation
Publication Date: 2001

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