Promega Corporation

Developmental Validation of a Real-Time PCR Assay for the Simultaneous Quantification...

Developmental Validation of a Real-Time PCR Assay for the Simultaneous Quantification of Total Human and Male DNA

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Abstract

Multiplex human short tandem repeat analysis demands reliable DNA quantification to consistently produce interpretable genotypes. The Plexor® HY System is a multiplex quantitative PCR assay to quantify total human and male DNA. We performed developmental validation of the Plexor® HY System to demonstrate the performance capabilities and limitations of the assay for forensic applications. Validation studies examined: a) human specificity, b) sensitivity, c) quantification of degraded DNA, d) impact of inhibitors, e) male/female mixture and Y-assay male specificity, f) reproducibility and concordance and g) population studies.

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