PowerPlex® CS7 System, Custom, Technical Manual

Literature # TMD030

The PowerPlex® CS7 System, Custom, allows co-amplification and three-color detection of seven STR loci, including LPL, F13B, FESFPS, F13A01, Penta D, Penta C and Penta E. One primer for each of the LPL, F13B, PESFPS, F13A01, and Penta D loci is labeled with fluorescein (FL); one primer for the Penta E locus is labeled with carboxy-tetramethylrhodamine (TMR); and one primer for the Penta C locus is labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy-fluorescein (JOE). All seven loci are amplified simultaneously in a single tube and analyzed in a single injection. The PowerPlex® CS7 System, Custom contains two loci, Penta D and Penta E, which overlap with the loci included in the PowerPlex® 16 HS System. This feature allows the PowerPlex® CS7 System, Custom to be used as a confirmatory kit in paternity applications using the five unshared STR loci to supplement the genotype and increase the available information.