PowerPlex® Matrix Standards, 310/377 Technical Bulletin

Literature # TBD018

Proper generation of a matrix file is critical to evaluate multicolor systems with the ABI PRISM® 310 Genetic Analyzer and ABI PRISM® 377 DNA Sequencer. The PowerPlex® Matrix Standards, 310/377, is used with the PowerPlex® 1.2 System or any of the GenePrint® Fluorescent STR Systems (fluorescein-labeled).

To prepare a matrix, four standards are run under the same capillary electrophoresis (CE) or gel conditions that are used for samples and allelic ladders. The PowerPlex® Matrix Standards, 310/377, consists of DNA fragments labeled with four different fluorescent dyes: one tube contains DNA fragments labeled with fluorescein, one tube contains DNA fragments labeled with carboxy-tetramethylrhodamine (TMR), two tubes contain DNA fragments labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy -fluorescein (JOE), and one tube contains DNA fragments labeled with carboxy-X-rhodamine (CXR). A matrix should be generated for each individual instrument.