NGF Emax® ImmunoAssay System Technical Bulletin
Literature # TB226
The NGF Emax® ImmunoAssay System is designed for sensitive and specific detection of NGF (nerve growth factor)in an antibody sandwich format. In this format, flat-bottom 96-well plates are coated with Anti-NGF Polyclonal Antibody (pAb), which binds soluble NGF. The captured NGF is bound by a second specific monoclonal antibody (mAb). After washing, the amount of specifically bound mAb is detected using a species-specific antibody conjugated to horseradish peroxidase (HRP) as a tertiary reactant. The unbound conjugate is removed by washing, and following an incubation with a chromogenic substrate, the color change is measured. The amount of NGF in the test solutions is proportional to the color generated in the oxidation-reduction reaction. Using this system, NGF in tissue culture supernatants or tissue extracts can be quantitated.
The NGF Emax® ImmunoAssay System offers several benefits:
- Specificity: Specific detection of NGF; typically less than 3%
cross-reactivity with other neurotrophic factors at 10ng/ml.
- Sensitivity: Detects a minimum of 7.8pg/ml of NGF.
- Flexibility: Available in sizes for two or five 96-well ELISA
plates; can configure plates as desired.
- High Value: Optimized reagents and protocol.
Printed in USA. Revised 3/07.