FastBreak™ Cell Lysis Reagent, 10X is a new cell lysis buffer for use with protein purification systems. The FastBreak™ Reagent can be added directly to E. coli cultures for gentle, efficient lysis in 10 to 15 minutes without the need for centrifugation or mechanical disruption. We demonstrate the compatibility of the FastBreak™ Buffer with the MagneHis™ Protein Purification System in both manual and robotic formats.
Promega Notes 86, 23–24.
Jude Stevens and Gary Kobs