The use of T vectors can greatly facilitate the cloning of PCR products. In this update, we describe a new version of the pGEM®-T Vector available in the pGEM®-T Easy Vector Systems. The pGEM®-T Easy Vector is produced by modifying the multiple cloning site (MCS) of the original pGEM®-T Vector so that a single restriction digestion with either Not I or EcoR I may be performed to remove the insert DNA. We also describe ways to optimize the results obtained using the pGEM®-T and pGEM®-T Easy Vector Systems.
Promega Notes 58, 36.
Liz Marcus, Jim Hartnett and Douglas R. Storts