Promega Corporation

psiCHECK™-1 and psiCHECK™-2 Vectors

The psiCHECK™-1 and psiCHECK™-2 Vectors are designed to provide a quantitative and rapid approach for initial optimization of RNA interference (RNAi). The vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. In both vectors Renilla luciferase is used as the primary reporter gene, and the gene of interest is cloned into a multiple cloning region located downstream of the Renilla translational stop codon. Initiation of the RNAi process by synthetic siRNAs or in vivo-expressed shRNAs toward a gene of interest results in cleavage and subsequent degradation of the fusion mRNA. M...

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psiCHECK™-1 Vector

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psiCHECK™-1 Vector

 Components

  • psiCHECK™-1 Vector

    C801A1 x 20μg
20μg
- C8011 zł 1 617,00 Add to cart

psiCHECK™-2 Vector

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psiCHECK™-2 Vector

 Components

  • psiCHECK™-2 Vector

    C802A1 x 20μg
20μg
- C8021 zł 1 617,00 Add to cart


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GenBank®/EMBL Accession Number

psiCHECK™-1 Accession Number AY535006; psiCHECK™-1 Vector sequence text file.
psiCHECK™-2 Accession Numbers AY535007; psiCHECK™-2 Vector sequence text file.

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

Mechanism of action of the psiCHECK Vectors.Figure 1. Mechanism of action of the psiCHECK™ Vectors.
psiCHECK-1 Vector.Figure 2. psiCHECK™-1 Vector.
psiCHECK-2 Vector.Figure 3. psiCHECK™-2 Vector.

Use Restrictions

C8011, C8021 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

C8011, C8021 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE LABEL LICENSE. If the researcher is not willing to accept the terms of this label license, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. "Commercial use" means any and all uses of this product and derivatives by a party for money or other consideration and may include but is not limited to use in: (1) product manufacture; and (2) to provide a service, information or data; and/or resale of the product or its derivatives, whether or not such product or derivatives are resold for use in research. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the luciferase gene except that researchers may: (1) create fused gene sequences provided that the coding sequence of the resulting luciferase gene has no more than four deoxynucleotides missing at the affected terminus compared to the intact luciferase gene sequence, and (2) insert and remove nucleic acid sequences in splicing research predicated on the inactivation or reconstitution of the luminescence of the encoded luciferase. No other use or transfer of this product or derivatives is authorized without the prior express written consent of Promega. In addition, researchers must either: (1) use luminescent assay reagents purchased from Promega for all determinations of luminescence activity of this product and its derivatives; or (2) contact Promega to obtain a license for use of the product and its derivatives. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this label license shall be governed under the laws of the State of Wisconsin, USA. This label license relates to Promega patents and/or patent applications on improvements to the luciferase gene.

C8011, C8021 U.S. Pat. No. 8,008,006 and European Pat. No. 1341808.

C8021 U.S. Pat. No. 5,670,356.

C8011, C8021 Certain applications of this product may require licenses from others.

C8021 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673. A license (from Promega for research reagent products and from The Regents of the University of California for all other fields) is needed for any commercial sale of nucleic acid contained within or derived from this product.

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