Promega Corporation

ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay

The ONE-Glo™ + Tox Assay combines luciferase assay chemistry with a cell viability marker to better understand reporter gene expression in the context of cell health. The assay uses a two-step, addition-only process to make these measurements in a single well of a plate, negating the need to run parallel assays.

The first part of the assay is a nonlytic fluorescence assay (CellTiter-Fluor™ Cell Viability Assay) that measures the relative number of live cells in a culture population after experimental manipulation. The CellTiter-Fluor™ Assay measures a conserved and constitutive protease activity wit...

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ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay

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ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay

 Components

  • ONE-Glo™ Luciferase Assay Buffer

    E605A1 x 10ml
  • ONE-Glo™ Luciferase Assay Substrate

    E606A1 x 1 vial
  • GF-AFC Substrate

    G608A1 x 10μl
  • Assay Buffer

    G610A1 x 10ml
1 plate
- E7110 zł 616,00 Add to cart

ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay

Close Window

ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay

 Components

  • ONE-Glo™ Luciferase Assay Buffer

    E605B1 x 100ml
  • ONE-Glo™ Luciferase Assay Substrate

    E606B1 x 1 vial
  • GF-AFC Substrate

    G608B2 x 50μl
  • Assay Buffer

    G610B1 x 50ml
10 plates
- E7120 zł 4 899,00 Add to cart


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Storage Conditions

Store the ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay components at –20°C. Please refer to the Technical Manual for other storage options.

For product intended use please see Patents & Disclaimers tab.

ONE-Glo™+Tox Assay_10471MAFigure 1. Schematic of the ONE-Glo™+Tox Luciferase Reporter and Cell Viability Assay.
Schematic diagram of the ONE-Glo + Tox Luciferase Reporter and Cell Viability Assay.Figure 2. Schematic diagram of the ONE-Glo + Tox Luciferase Reporter and Cell Viability Assay.
Ionomycin titration in standard 96- and 384-well plate formats.Figure 3. Ionomycin titration in standard 96- and 384-well plate formats.

1 × 104 (96-well plate; Panel A) or 5 × 103 (384-well plate; Panel B) cells expressing NFAT response element were treated with serial titrations of ionomycin in the presence of PMA for 6 hours following the example protocols in Section 4.B and 4.C of the Technical Manual. At specific concentrations, ionomycin and PMA work cooperatively to stimulate NFAT-dependent gene expression. However, higher concentrations of ionomycin result in cytotoxicity seen as a decrease in viability (fluorescence). A decrease in reporter expression (luciferase) activity is also observed due to the increase in cytotoxicity.

1 × 104 (96-well plate; Panel A) or 5 × 103 (384-well plate; Panel B) cells expressing NFAT response element were treated with serial titrations of ionomycin in the presence of PMA for 6 hours following the example protocols in Section 4.B and 4.C of the Technical Manual. At specific concentrations, ionomycin and PMA work cooperatively to stimulate NFAT-dependent gene expression. However, higher concentrations of ionomycin result in cytotoxicity seen as a decrease in viability (fluorescence). A decrease in reporter expression (luciferase) activity is also observed due to the increase in cytotoxicity.

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Use Restrictions

E7110, E7120 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

E7110, E7120 U.S. Pat. Nos. 7,416,854, 7,553,632 and other patents pending.

E7110, E7120 Patent Pending.

E7110, E7120 Certain applications of this product may require licenses from others.

E7110, E7120 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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