Promega Corporation

ProTEV Plus

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ProTEV Plus is an improved 48kDa version of the Nla protease from tobacco etch virus (TEV) that has been engineered to be more stable than native TEV protease for prolonged enzymatic activity. It is a highly specific proteolytic enzyme that cleaves within a seven-amino-acid sequence (ENLYFQ(G/S)). ProTEV Plus is active over a wide range of pH values (5.5–8.5) and temperatures (4–30°C). It can be used to cleave protein fusions that have been engineered with the above amino acid sequence at the desired cleavage site. The enzyme is compatible for both in-solution and on-column cleavage reactions...

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

ProTEV Plus

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ProTEV Plus

 Components

  • DTT

    P117B1 x 250μl
  • ProTEV Buffer, 20X

    V602A1 x 1ml
  • ProTEV Plus

    V610A1 x 1,000u
1,000u
5u/μl V6101 zł 503,00 Add to cart

ProTEV Plus

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ProTEV Plus

 Components

  • DTT

    P117C1 x 1.25ml
  • ProTEV Buffer, 20X

    V602B1 x 8ml
  • ProTEV Plus

    V610B1 x 8,000u
8,000u
5u/μl V6102 zł 3 524,00 Add to cart


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Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

Coomassie-stained gel of ProTEV Plus showing 1 unit (U) of protein with the specific activity of 1 unit/µg.Figure 1. Coomassie®-stained gel of ProTEV Plus showing 1 unit (U) of protein with the specific activity of 1 unit/µg.
Cleavage of 20µg of GST-MBP fusion protein with ProTEV protease after 60 minutes at 30°C.Figure 2. Cleavage of 20µg of GST-MBP fusion protein with ProTEV protease after 60 minutes at 30°C.

Use Restrictions

V6101, V6102 For Research Use Only. Not for Use in Diagnostic Procedures.

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