Promega Corporation

Glycosidases

Endoglycosidase H (Endo H) is a recombinant glycosidase cloned from Streptomyces plicatus and overexpressed in E. coli. Endo H cleaves the chitobiose core of high mannose and a limited number of hybrid oligosaccharides from N-linked glycoproteins. It does not cleave complex glycans. Enzymatic cleavage is between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, leaving one N-acetylglucosamine residue on the asparagine. This is in contrast to PNGase F, which cleaves all asparagine-linked oligosaccharides.

Unit Definition: One unit is defined as the amount of enzyme required to...

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Endo H
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Endo H  Components

  • Endo H

    V487A1 x 10,000 units
  • 10X Endo H Reaction Buffer

    V490A1 x 1ml
  • 10X Denaturing Solution

    V492A1 x 1ml
10,000 units
500u/μl V4871 zł 260,00 Add to cart
Endo H
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Endo H  Components

  • Endo H

    V487B1 x 50,000 units
  • 10X Endo H Reaction Buffer

    V490A1 x 1ml
  • 10X Denaturing Solution

    V492A1 x 1ml
50,000 units
500u/μl V4875 zł 1 040,00 Add to cart
Protein Deglycosylation Mix
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Protein Deglycosylation Mix  Components

  • 10X Denaturing Solution

    V492A1 x 1ml
  • Protein Deglycosylation Mix

    V493A1 x 100μl
  • 10X Deglycosylation Reaction Buffer

    V494A1 x 1ml
  • 10% NP-40

    V495A1 x 1ml
  • Fetuin

    V496A1 x 50μl
20 reactions
- V4931 zł 1 907,00 Add to cart
Fetuin
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Fetuin  Components

  • Fetuin

    V496A1 x 50μl
500μg
10mg/ml V4961 zł 303,00 Add to cart

PNGase F

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PNGase F

 Components

  • PNGase F

    V483A1 x 500 units
500 units
10u/μl V4831 zł 558,00 Add to cart


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Storage Conditions

Store Endo H and Fetuin at –30 to –10°C. Store Protein Deglycosylation Mix at 2–10°C.

Unit Definition

Endo H: One unit is defined as the amount of enzyme required to remove >95% of the carbohydrate from 10μg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10μl.

For product intended use please see Patents & Disclaimers tab.

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Use Restrictions

V4871, V4875, V4931, V4961, V4831 For Research Use Only. Not for Use in Diagnostic Procedures.

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