Sample Types Processed Using the PureYield™ RNA Midiprep System
RNA from the following sample types has been successfully purified at Promega or by external laboratories using the PureYield™ RNA Midiprep System.
Sample Types
Many of these protocols have been communicated to Promega by external laboratories. Because these protocols have not been performed at Promega, they are intended to be guidelines that might require optimization.
This list will be updated as additional information becomes available. Contact Promega Technical Services for the latest information on available protocols.
Sample Types Processed Using the PureYield™ RNA Midiprep System.
| Sample Type |
Promega |
External |
Comments |
| Blood and blood cells |
|
|
A general protocol for total RNA isolation from blood cells is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resources are listed below. |
| Human blood |
Yes |
|
A protocol is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9. |
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| Sample Type |
Promega |
External |
Comments |
| Mammalian tissues |
|
|
A general protocol for total RNA isolation from tissue is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resources are listed below. |
| Rat liver |
Yes |
|
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9. |
| Rat lung |
Yes |
|
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9. |
| Rat kidney |
Yes |
|
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9. |
| Rat spleen |
Yes |
|
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9. |
| Rat brain |
Yes |
|
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9. |
| Rat heart |
Yes |
|
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9. |
| Rat muscle |
Yes |
|
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9. |
| Bovine liver |
Yes |
|
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279. |
| Bovine spleen |
Yes |
|
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279. |
| Bovine heart |
Yes |
|
Follow the standard protocol for tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279. |
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| Sample Type |
Promega |
External |
Comments |
| Plant tissues |
|
|
A general protocol for total RNA isolation from plant tissue is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resources are listed below. |
| Canola leaf |
Yes |
|
Follow the standard protocol for plant tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9. |
| Corn/maize leaf, stem and silk tissue |
Yes |
Yes |
Follow the standard protocol for plant tissue in the PureYield™ RNA Midiprep System Technical Manual #TM279. |
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| Sample Type |
Promega |
External |
Comments |
| Cultured cells |
Yes |
|
A general protocol for total RNA isolation from mammalian cultured cells is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279. |
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| Sample Type |
Promega |
External |
Comments |
| Bacteria |
|
|
A general protocol for total RNA isolation from Gram-positive and Gram-negative bacteria is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resources are listed below. |
| Escherichia coli |
Yes |
|
Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9. |
| Bacillus subtilis |
Yes |
|
Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279. |
| Bacillus sphaericus |
Yes |
|
Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279. |
| Bacillus globigii |
Yes |
|
Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279. |
| Artherobacter luteus |
Yes |
|
Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279. |
| Micrococcus luteus |
Yes |
|
Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279. |
| Staphylococcus aureus |
Yes |
|
Follow the standard protocol for bacteria in the PureYield™ RNA Midiprep System Technical Manual #TM279. |
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| Sample Type |
Promega |
External |
Comments |
| Other organisms and tissues |
|
|
|
| Saccharomyces cerevisiae |
Yes |
|
A protocol is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Brisco, P. et al. (2006) Promega Notes 92, 14–9. |
| Chicken liver |
Yes |
|
Follow the standard protocol in the PureYield™ RNA Midiprep System Technical Manual #TM279. |
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| Sample Type |
Promega |
External |
Comments |
| Miscellaneous |
|
|
|
| phenol:chloroform-extracted RNA samples |
Yes |
|
A protocol for clean up for phenol:chloroform-extracted RNA samples is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279.
Additional resource:
Mandrekar, M. et al. (2006) Promega Notes 94, 7–11. |
| RNA pellet from an alcohol precipitation |
Yes |
|
A protocol for clean up of RNA pellets is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279. |
| Purified RNA in an aqueous solution |
Yes |
|
A protocol for cleaning up RNA in an aqueous solution is provided in the PureYield™ RNA Midiprep System Technical Manual #TM279. |
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