Kazusa DNA Research Institute (KDRI)
KDRI is an academic Japanese research institute dedicated to genome-scale studies and the function of individual genes. The Kazusa ORFeome Project was initiated to create a collection of human and mouse ORFs to enable the larger research community with a readily accessible, highly validated ORFeome. KDRI is an active member of the global ORFeome Collaboration, and are experts in HUGE (Human Unidentified Gene-Encoded) large proteins (>4kb). Promega and KDRI have established a partnership, allowing Promega customers access to Kazusa's coveted collection of experimentally validated clones.
Experimentally-Validated Collection
Kazusa understands the value of extensive validation, and provides this data to their customers for the HaloTag® ORF collection. Check out the representative data pieces below, which are available for nearly all HaloTag®-fusion ORFs before you order.
Fluorescent Microscopy demonstrating in situ labeling and visualization of HaloTag® fusions with HaloTag® TMR Ligand.
Expression Validation by SDS-PAGE analysis of expressed HaloTag®-fusions in HEK293 cell lysates
Insert Size Confirmation through agarose gel electrophoresis
Sequence Validation of both the 5' and 3' end sequences by single-pass sequencing
Overview of the Collection
| Features |
HaloTag® Collection
PID beginning with FHC |
Flexi® Collection
PID beginning with FXC |
| Size of Collection |
8,872 human clones |
6,396 human clones |
| Fusion Tag |
HaloTag® for protein purification, imaging and pull-downs |
Un-tagged |
| Validated Clones |
| Sequence Validated |
✓ 100% clones |
✓ |
| Insert Validated |
✓ 99.8% clones |
✓ 96% clones |
| Expression Validated |
✓ 99.4% clones |
|
| Localization Validated |
✓ 77.91% clones |
|
| Format |
DNA |
DNA |
| Typical Delivery |
4-6 weeks |
4-6 weeks |
Gene Family Sets & Discounts Available
Allow us to quickly map your proteins of interest to the Kazusa collection to develop a custom set to meet your research needs. Send us an e-mail at proteomics@promega.com for pre-defined and custom gene family sets inquiries.
Gene Family Sets
- Tyrosine Kinases
- MAP Kinases
- G-Protein Coupled Receptors (G-PCRs)
- Phosphatases
- Transcription Factor-related Genes
- Membrane Proteins
- Protein Complexes
- Cytokines & Chemokines
- Ubiquitin Hydrolases (DUBS)
- Ubiquitin Ligases (E1, E2, E3)
- Tumor Suppressors
- Oncogenes
- Transporters and Pumps
- Proteases
Research Areas
- Epigenetics
- Differentiation and Stem Cells
- Cancer
- Regulation
- Signaling
- Cell Cycle
- Ion Channels
- Apoptosis
- DNA Repair
- RNAi
Discounts are available for larger orders (excluded from additional promotions)
- 10% discount by ordering 20-49 clones
- 20% discount by ordering more than 50 clones
Inquire at proteomics@promega.com before placing an order.
