Promega Corporation

Assay Principle

Glutathione is essential for detoxification of reactive oxygen species and xenobiotics. Measuring changes in total glutathione (GSH +GSSG) and oxidized glutathione (GSSG) is important for assessing toxicological responses that can promote oxidative stress and lead to apoptosis and cell death. The GSH/GSSG-Glo™ Assay allows you to directly measure GSSG and total glutathione for determination of GSH/GSSG ratios with a simple, sensitive, add-mix-measure protocol incorporating Promega's superior Ultra-Glo™ Luciferase bioluminescent technology.


Prepare one set of wells for total (GSH +GSSG) and one set of wells for GSSG measurement

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