Promega Corporation

SwabSolution™ Kit, PunchSolution™ Kit &
5X AmpSolution™ Reagent

Rapid Processing Solutions for PowerPlex® Systems

Simplify Your Database or Paternity Workflow With No Reduction in STR Performance

SwabSolution™, PunchSolution™ and AmpSolution™ products streamline the processing of swabs and punches for PowerPlex® STR analysis without compromising your results. While direct amplification from FTA® cards is transforming workflows in databasing and paternity testing labs, buccal swabs and non FTA cards are not ideal substrates for direct amplification. The Promega SwabSolution™ Kit, PunchSolution™ Kit and AmpSolution™ Reagent provide new, faster methods for processing swabs and card punches. Additionally, AmpSolution™ Reagent enables direct amplification from traditional PowerPlex® kits that were not originally intended for that application, minimizing the need to validate a new system to perform direct amplification.

  • Quickly and easily process fresh or old swabs with the SwabSolution™ Kit
  • Process nonFTA punches on the same plate with the PunchSolution™ Kit
  • Perform direct amplification with FTA® punches using your validated PowerPlex® Systems including PowerPlex® 16 HS, ESI and ESX Systems with the AmpSolution™ Reagent

Swab Analysis

The SwabSolution™ Kit works in tandem with PowerPlex® Systems, including PowerPlex® 21, 18D, 16 HS, ESX, ESI, CS7 and Y23 Systems to enable reproducible, robust STR profiles from swab buccal samples.

As demonstrated in the "Buccal Swab Workflow", when using SwabSolution™ Kit, centrifugation or washes are no longer needed to extract DNA from the swab head. Eliminating those steps offers streamlined workflows and expanded throughput capabilities. Furthermore, quantification of DNA is possible, as is long-term storage of SwabSolution™ extracts for future use.

SwabSolution™ Kit—Buccal Swab Workflow

Figure 10584MB

Swab Analysis, PowerPlex® 16 HS System

Figure 10591TA

An OmniSwab™ buccal sample was pretreated with SwabSolution™ Reagent as recommended in the SwabSolutionKit Technical Manual TMD037. Amplification was performed using the PowerPlex® 16 HS System. Amplified products were separated on an Applied Biosystems 3130xl Genetic Analyzer (3kV, 5-second injection).

Non FTA Punch Analysis

PunchSolution™ Kit works in conjunction with PowerPlex® Systems, including the PowerPlex® 21, 18D, 16HS, ESX, ESI, CS7 and Y23 Systems to provide robust amplification profiles from nonFTA cards. As shown in the "NonFTA Punch Workflow", the PunchSolution™ Kit offers a simple sample preparation process to simplify analysis. At 10µl, the volume of PunchSolution™ Reagent allows easier punch handling than other pretreatment products that only use 2µl of solution. Higher quality, easier-to-interpret profiles are obtained, compared to other methods for processing nonFTA punches.

PunchSolution™ Kit—NonFTA Punch Workflow

Figure 10583MB

Blood on S&S903, PowerPlex® 18D Amplification

Figure 10585TA

One 1.2mm blood punch on S&S903 card was pretreated with PunchSolution™ Reagent as recommended in the PunchSolutionKit Technical Manual TMD038. Amplification was performed using the PowerPlex® 18D System. Amplified products were separated on an Applied Biosystems 3130xl Genetic Analyzer (3kV, 5-second injection).

FTA® Punch Analysis

5X AmpSolution™ Reagent enables direct amplification from FTA® punches with many traditional PowerPlex® Systems, including the PowerPlex® 16 HS, ESX, ESI and CS7 Systems.

Buccal Samples on FTA® Punches, PowerPlex® ESX 17 System

Figure 10570MA

Two 1.2mm punches from a buccal sample on an FTA® card were amplified using the PowerPlex® ESX 17 System. Prior to amplification, AmpSolution™ Reagent was added to the reaction mix. Amplified products were separated on an Applied Biosystems 3130xl Genetic Analyzer (3kV, 5-second injection).

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