Promega Corporation

Reverse Transcription System

The Reverse Transcription System provides reagents to efficiently reverse transcribe RNA into cDNA in 15 minutes. The cDNA prepared from each reaction using this system may be used directly in multiple PCR amplifications using Taq DNA polymerase. The AMV Reverse Transcriptase synthesizes single-stranded cDNA from total or poly(A)+ RNA. Both Oligo(dT)15 and Random Primers are included, allowing cDNA synthesis from virtually any RNA source. The system contains sufficient reagents for 100 cDNA synthesis reactions, processing 1μg of RNA per reaction. Each cDNA synthesis reaction may be divided and used in up to 20 sepa...

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  • Prices valid for customers of Promega GmbH only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

Reverse Transcription System

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Reverse Transcription System

 Components

  • MgCl2

    A351H1 x 1.2ml
  • Reverse Transcription 10X Buffer

    A356A1 x 1.4ml
  • Oligo(dT)15 Primer

    C110B1 x 50μg
  • dNTP Mix

    C114B1 x 320μl
  • Random Primers

    C118B1 x 50μg
  • 1.2kb Kanamycin Positive Control RNA

    C138A1 x 5μg
  • AMV Reverse Transcriptase (HC)

    M900B1 x 1,500u
  • Recombinant RNasin® Ribonuclease Inhibitor

    N251A1 x 2,500u
  • Nuclease-Free Water

    P119E1 x 13ml
100 reactions
- A3500 zł 2 059,00 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

Magnesium Chloride Solution

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Magnesium Chloride Solution

 Components

  • MgCl2

    A351B2 x 750μl
1.5ml
25mM A3511 zł 134,00 Add to cart

Reverse Transcription 10X Buffer

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Reverse Transcription 10X Buffer

 Components

  • Reverse Transcription 10X Buffer

    A356A1 x 1.4ml
1.4ml
- A3561 zł 156,00 Add to cart

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Storage Conditions

Store at –20°C. Store Positive Control RNA at –70°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

A3500 For Research Use Only. Not for Use in Diagnostic Procedures.
A3511, A3561 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.

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