Anti-NGF mAb

Nerve growth factor (NGF) is a member of the neurotrophin family of growth factors. NGF is expressed in sympathetic and sensory-innervated peripheral tissues and mediates phosphorylation of specific intracellular proteins. At the cellular level, NGF expression has been demonstrated in lymphocytes, smooth muscle cells, epithelial cells, astrocytes, fibroblasts and Schwann cells. Anti-NGF mAb was designed as a specific marker of NGF in Western blotting, ELISA and immunostaining applications.

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Nerve growth factor (NGF) is a member of the neurotrophin family of growth factors. NGF is expressed in sympathetic and sensory-innervated peripheral tissues and mediates phosphorylation of specific intracellular proteins. At the cellular level, NGF expression has been demonstrated in lymphocytes, smooth muscle cells, epithelial cells, astrocytes, fibroblasts and Schwann cells. Anti-NGF mAb was designed as a specific marker of NGF in Western blotting, ELISA and immunostaining applications.

Features - Benefits

• Immunogen: Purified murine NGF, 2.5S.
• Antibody Form: Rat IgG (clone 1G3) provided at 1mg/ml as frozen liquid in PBS containing no preservatives.
• Specificity: Reacts with human NGF, 2.5S mNGF and to a lesser extent with 7S mNGF. Cross-reacts between mammalian species.
• Activity: The Anti-NGF mAb exhibits a half-maximal titer of less than or equal to 250ng/ml in an ELISA protocol using 100ng of 2.5S mNGF (Cat.# G5141).

Applications

(Suggested working concentration)

• Western blotting (1μg/ml).
• ELISA (0.5μg/ml).
• Immunohistochemistry (0.5–1μg/ml).
• Immunocytochemistry (0.5–1μg/ml).

For more information, see the Protocols & Applications Guide.

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