The PowerPlex® Y System allows co-amplification and three-color detection of twelve loci. The system contains primers for the loci DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. One primer specific for DYS389I/II, DYS391 and DYS439 is labeled with fluorescein (FL). One primer specific for each of the DYS385a/b, DYS390 and DYS393 loci is labeled with carboxy-tetramethylrhodamine (TMR). One primer specific for each of the DYS19, DYS392, DYS437 and DYS438 loci is labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy-fluorescein (JOE).
All twelve loci are amplified simultan...
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The PowerPlex® Y System allows co-amplification and three-color detection of twelve loci. The system contains primers for the loci DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS437, DYS438 and DYS439. One primer specific for DYS389I/II, DYS391 and DYS439 is labeled with fluorescein (FL). One primer specific for each of the DYS385a/b, DYS390 and DYS393 loci is labeled with carboxy-tetramethylrhodamine (TMR). One primer specific for each of the DYS19, DYS392, DYS437 and DYS438 loci is labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxy-fluorescein (JOE).
All twelve loci are amplified simultaneously in a single tube and analyzed in a single injection or gel lane. Fragment sizing is provided by an internal size standard (Internal Lane Standard 600) labeled with carboxy-X-rhodamine (CXR). Color deconvolution can be performed using matrix standards available from Promega.
The PowerPlex® Y System is compatible with the ABI PRISM® 310, 3100 and 3100-Avant and Applied Biosystems 3130 and 3130xl Genetic Analyzers and ABI PRISM® 377 DNA Sequencer.
Features - Benefits
- Amplification of 12 Y-STR Loci in One Reaction: Amplify the nine loci defined as the European minimal haplotype and endorsed by ISFG (International Society of Forensic Genetics)* plus the two loci added to this panel by SWGDAM (Scientific Working Group on DNA Analysis Methods).
Note: The data in the PowerPlex® Y Haplotype Database have been added to the US Y-STR Database, a searchable listing of 11- to 17-locus Y-STR haplotypes. For Y-STR haplotype searches, visit the US Y-STR Database at: www.usystrdatabase.org or Y-Chromosome Haplotype Reference Database at: www.yhrd.org
*ISFG-endorsed loci are DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393. SWGDAM-endorsed loci are DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392, DYS393, DYS438 and DYS439.
- Maximum Sensitivity: The system is optimized for use with 0.5–1ng of template DNA. However, each lot is performance tested to provide reproducible results with 0.25ng of DNA. Additional studies show interpretable results can be obtained with <0.25ng of DNA.
- Superior Specificity—You Amplify Only Male DNA: Each lot is performance tested to ensure no amplification with up to 100ng of female DNA. Additional studies show no amplification with >100ng of female DNA. This makes the PowerPlex® Y System a great choice to analyze "heavy" mixture samples.
- Short PCR Products: Amplification products are all less than 335 bases, which increases the likelihood of obtaining a full profile from degraded samples.
- No Locus Overlap: Primers were designed so that alleles for each locus do not overlap with a neighboring locus, reducing the likelihood of misinterpretation.
- Typing of Samples with Greater Confidence: Allelic ladders for all 12 loci are provided to simplify interpretation through the use of the PowerTyper™ Macro. The wide span of alleles included in the ladder allows typing of more rare alleles.
- Automatic Assignment of Genotypes: The PowerTyper™ Y Macro (available separately) automatically labels fragments from Genotyper® data. The PowerTyper™ Macros can be downloaded or ordered on CD-ROM. Panels and bins text files for use with GeneMapper® ID are available for download.
Applications
For more information, see the Protocols & Applications Guide.
Notes
The PowerPlex® Matrix Standards, 310 (Cat.# DG4640), is required for use of this system with the ABI PRISM® 310 Genetic Analyzer. The PowerPlex® Matrix Standards, 3100/3130 (Cat.# DG4650), is required for use of this system with the ABI PRISM® 3100 and 3100-Avant and Applied Biosystems 3130 and 3130xl Genetic Analyzers.
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