Promega Corporation

Proteasome-Glo™ Assays

The Proteasome-Glo™ Assays are homogeneous, luminescent assays that individually measure the chymotrypsin-like, trypsin-like and caspase-like protease activities associated with the proteasome in a purified enzyme-based format. The 26S proteasome is a 2.5MDa multiprotein complex found in all eukaryotic cells. Adding the Proteasome-Glo™ Cell-Based Reagent in an "add-mix-measure" format results in proteasome cleavage of the substrate and rapid generation of a luminescent signal produced by the luciferase reaction.

The three luminogenic substrates used to monitor specific protease activities include: Suc-LLVY-aminol...

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Product Size Conc. Catalog # *List Price Order QTY Add to Cart

Proteasome-Glo™ Chymotrypsin-Like Assay

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Proteasome-Glo™ Chymotrypsin-Like Assay

 Components

  • Proteasome-Glo™ Buffer

    G854A1 x 10ml
  • Suc-LLVY-Glo™ Substrate

    G856C1 x 50μl
  • Luciferin Detection Reagent

    V859A1 x 1 each
10ml
- G8621 zł 1 517,00 Add to cart

Proteasome-Glo™ Chymotrypsin-Like Assay

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Proteasome-Glo™ Chymotrypsin-Like Assay

 Components

  • Proteasome-Glo™ Buffer

    G854B1 x 50ml
  • Suc-LLVY-Glo™ Substrate

    G856D1 x 250μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
50ml
- G8622 zł 5 280,00 Add to cart

Proteasome-Glo™ Trypsin-Like Assay

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Proteasome-Glo™ Trypsin-Like Assay

 Components

  • Proteasome-Glo™ Buffer

    G854A1 x 10ml
  • Z-LRR-Glo™ Substrate

    G855A1 x 100μl
  • Luciferin Detection Reagent

    V859A1 x 1 each
10ml
- G8631 zł 1 517,00 Add to cart

Proteasome-Glo™ Trypsin-Like Assay

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Proteasome-Glo™ Trypsin-Like Assay

 Components

  • Proteasome-Glo™ Buffer

    G854B1 x 50ml
  • Z-LRR-Glo™ Substrate

    G855B1 x 500μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
50ml
- G8632 zł 5 280,00 Add to cart

Proteasome-Glo™ Caspase-Like Assay

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Proteasome-Glo™ Caspase-Like Assay

 Components

  • Proteasome-Glo™ Buffer

    G854A1 x 10ml
  • Z-nLPnLD-Glo™ Substrate

    G857A1 x 50μl
  • Luciferin Detection Reagent

    V859A1 x 1 each
10ml
- G8641 zł 1 517,00 Add to cart

Proteasome-Glo™ Caspase-Like Assay

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Proteasome-Glo™ Caspase-Like Assay

 Components

  • Proteasome-Glo™ Buffer

    G854B1 x 50ml
  • Z-nLPnLD-Glo™ Substrate

    G857B1 x 250μl
  • Luciferin Detection Reagent

    V859B1 x 1 each
50ml
- G8642 zł 5 280,00 Add to cart

Proteasome-Glo™ 3-Substrate System

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Proteasome-Glo™ 3-Substrate System

 Components

  • Proteasome-Glo™ Chymotrypsin-Like Assay

    G86211 x 10ml
  • Proteasome-Glo™ Trypsin-Like Assay

    G86311 x 10ml
  • Proteasome-Glo™ Caspase-Like Assay

    G86411 x 10ml
10ml
- G8531 zł 3 130,00 Add to cart

Proteasome-Glo™ 3-Substrate System

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Proteasome-Glo™ 3-Substrate System

 Components

  • Proteasome-Glo™ Chymotrypsin-Like Assay

    G86221 x 50ml
  • Proteasome-Glo™ Trypsin-Like Assay

    G86321 x 50ml
  • Proteasome-Glo™ Caspase-Like Assay

    G86421 x 50ml
50ml
- G8532 zł 8 960,00 Add to cart


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Storage Conditions

Store the Proteasome-Glo™ Assay components at –20°C.

For product intended use please see Patents & Disclaimers tab.

The luminogenic substrates containing the Suc-LLVY, Z-LRR or Z-nLPnLD sequence are recognized by the 20S proteasome.Figure 1. The luminogenic substrates containing the Suc-LLVY, Z-LRR or Z-nLPnLD sequence are recognized by the 20S proteasome.
Luminescence is proportional to proteasome concentration.Figure 2. Luminescence is proportional to proteasome concentration.
Luminescent proteasome assays are more sensitive than fluorescent proteasome assays.Figure 3. Luminescent proteasome assays are more sensitive than fluorescent proteasome assays.

Use Restrictions

G8621, G8622, G8631, G8632, G8641, G8642, G8531, G8532 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G8621, G8622, G8631, G8632, G8641, G8642, G8531, G8532 Patent Pending.

G8621, G8622, G8631, G8632, G8641, G8642, G8531, G8532 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

G8621, G8622, G8631, G8632, G8641, G8642, G8531, G8532 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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