Promega Corporation

cAMP-Glo™ Max Assay

The cAMP-Glo™ Max Assay is a homogeneous, bioluminescent and high-throughput assay to measure cyclic AMP (cAMP) levels in cells. Compounds that modulate GPCRs coupled with adenylate cyclase typically alter intracellular cAMP levels. The cAMP-Glo™ Max Assay monitors cAMP levels in cells in response to the effect of agonists, antagonists or test compounds on G protein-coupled receptors (GPCRs). The assay is based on the principle that cyclic AMP (cAMP) stimulates protein kinase A (PKA) holoenzyme activity, decreasing available ATP and leading to decreased light production in a coupled luciferase reaction.

T...

Expand to Read More »

  • Share
  • Print
  • Email
  • Prices valid for customers of Promega GmbH only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

cAMP-Glo™ Max Assay

Close Window

cAMP-Glo™ Max Assay

 Components

  • Protein Kinase A

    V135A1 x 20μl
  • cAMP-Glo™ ONE Buffer

    V166A1 x 2.5ml
  • MgCl2, 1M

    V330A1 x 1ml
  • cAMP, 1mM

    V642B1 x 100μl
  • Kinase-Glo® Substrate

    V672A1 x 1 vial
  • Kinase-Glo® Buffer

    V673A1 x 10ml
2 plates
- V1681 zł 2 046,00 Add to cart

cAMP-Glo™ Max Assay

Close Window

cAMP-Glo™ Max Assay

 Components

  • Protein Kinase A

    V135B1 x 200μl
  • cAMP-Glo™ ONE Buffer

    V166B1 x 20ml
  • MgCl2

    V330B1 x 5ml
  • cAMP, 1mM

    V642A1 x 500μl
  • Kinase-Glo® Substrate

    V672B1 x 1 vial
  • Kinase-Glo® Buffer

    V673B1 x 100ml
20 plates
- V1682 Please Enquire

cAMP-Glo™ Max Assay

Close Window

cAMP-Glo™ Max Assay

 Components

  • Protein Kinase A

    V135B10 x 200μl
  • cAMP-Glo™ ONE Buffer

    V166B10 x 20ml
  • MgCl2

    V330B4 x 5ml
  • cAMP, 1mM

    V642A6 x 500μl
  • Kinase-Glo® Substrate

    V672B10 x 1 vial
  • Kinase-Glo® Buffer

    V673B10 x 100ml
10 × 20 plates
- V1683 Please Enquire


You Also May Be Interested In

Storage Conditions

Store the system at –20°C. Before use, completely thaw all components at room temperature, except for the Protein Kinase A, which should be kept on ice when not at –20°C. After thawing, mix all components thoroughly before use. Once prepared, the cAMP detection solution (cAMP-Glo™ ONE Buffer with Protein Kinase A) should not be frozen. Once prepared, the Kinase-Glo® Reagent should be dispensed into aliquots and stored at –20°C. See the product label for the expiration date.

For product intended use please see Patents & Disclaimers tab.

Representation of cAMP production in cells and the cAMP-Glo and cAMP-Glo Max Assays.Figure 1. Schematic diagram of cAMP production in cells and the cAMP-Glo™ Max Assay.

/~/media/images/resources/figures/6100-6199/6127ma_550px.jpg?la=pl-PL

Use Restrictions

V1681, V1682, V1683 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V1681, V1682, V1683 U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

V1681, V1682, V1683 U.S. Pat. No. 7,700,310 and other patents and patents pending.

V1681, V1682, V1683 U.S. Pat. No. 7,741,067 and other patents and patents pending.

V1681, V1682, V1683 Patent Pending.

V1681, V1682, V1683 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

V1681, V1682, V1683 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

Prefer a different language?

Your country is set to Poland. Your language is set to polski. Please select the language that will best suit your needs:

This is correct, continue to site »

I need additional help

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.