Promega Corporation

cAMP-Glo™ Assay

The cAMP-Glo™ Assay is a homogeneous, bioluminescent and high-throughput assay for measuring cAMP levels in cells. The cAMP-Glo™ Assay monitors cAMP production in cells in response to the effects of test compounds on G protein-coupled receptors (GPCR). GPCRs that couple with adenylate cyclase will increase or decrease intracellular cAMP. The assay is based on the principle that cyclic AMP (cAMP) stimulates protein kinase A (PKA) holoenzyme activity, decreasing available ATP and leading to decreased light production in a coupled luciferase reaction.

The cAMP-Glo™ Assay can be performed in 96-, 384- or 1536-well ...

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cAMP-Glo™ Assay

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cAMP-Glo™ Assay

 Components

  • Protein Kinase A

    V135D1 x 16μl
  • cAMP-Glo™ Lysis Buffer

    V151A1 x 5ml
  • cAMP-Glo™ Reaction Buffer

    V152A1 x 8ml
  • cAMP, 1mM

    V642B1 x 100μl
  • Kinase-Glo® Substrate

    V672A1 x 1 vial
  • Kinase-Glo® Buffer

    V673A1 x 10ml
300 assays
(384-well plate)
- V1501 zł 997,00 Add to cart

cAMP-Glo™ Assay

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cAMP-Glo™ Assay

 Components

  • Protein Kinase A

    V135C1 x 160μl
  • cAMP-Glo™ Lysis Buffer

    V151B1 x 25ml
  • cAMP-Glo™ Reaction Buffer

    V152B1 x 50ml
  • cAMP, 1mM

    V642B1 x 100μl
  • Kinase-Glo® Substrate

    V672B1 x 1 vial
  • Kinase-Glo® Buffer

    V673B1 x 100ml
3,000 assays
(384-well plate)
- V1502 zł 6 507,00 Add to cart

cAMP-Glo™ Assay

Close Window

cAMP-Glo™ Assay

 Components

  • Protein Kinase A

    V135C10 x 160μl
  • cAMP-Glo™ Lysis Buffer

    V151C1 x 250ml
  • cAMP-Glo™ Reaction Buffer

    V152C1 x 500ml
  • cAMP, 1mM

    V642A1 x 500μl
  • Kinase-Glo® Substrate

    V672B10 x 1 vial
  • Kinase-Glo® Buffer

    V673B10 x 100ml
30,000 assays
(384-well plate)
- V1503 Please Enquire


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Storage Conditions

Store the system at –20°C. Once prepared, the cAMP detection solution (cAMP-Glo™ Reaction Buffer with Protein Kinase A) should not be frozen. Once prepared, the Kinase-Glo® Reagent should be dispensed into aliquots and stored at –20°C. See the product label for the expiration date.

For product intended use please see Patents & Disclaimers tab.

Representation of cAMP production in cells and the cAMP-Glo and cAMP-Glo Max Assays.Figure 1. Representation of cAMP production in cells and the cAMP-Glo™ Assay.

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Use Restrictions

V1501, V1502, V1503 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V1501, V1502, V1503 U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

V1501, V1502, V1503 U.S. Pat. No. 7,700,310 and other patents and patents pending.

V1501, V1502, V1503 U.S. Pat. No. 7,741,067 and other patents and patents pending.

V1501, V1502, V1503 Patent Pending.

V1501, V1502, V1503 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

V1501, V1502, V1503 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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