Promega Corporation

T4 DNA Ligase

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T4 DNA Ligase catalyzes the joining of two strands of DNA between the 5´-phosphate and the 3´-hydroxyl groups of adjacent nucleotides in either a cohesive-ended or blunt-ended configuration. The enzyme has also been shown to catalyze the joining of RNA to either a DNA or RNA strand in a duplex molecule but will not join single-stranded nucleic acids.

The T4 DNA Ligase Buffer Pack includes 3 tubes of T4 DNA Ligase 10X Reaction Buffer. The composition of the 10X reaction buffer is 300mM Tris-HCl (pH 7.8 at 25°C), 100mM MgCl2, 100mM DTT and 10mM ATP.

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  • Prices valid for customers of Promega GmbH only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

T4 DNA Ligase

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T4 DNA Ligase

 Components

  • T4 DNA Ligase 10X Buffer

    C126B1 x 200μl
  • T4 DNA Ligase

    M180A1 x 100u
100u
(Weiss units)
1–3u/μl M1801 zł 108,00 Add to cart

T4 DNA Ligase

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T4 DNA Ligase

 Components

  • T4 DNA Ligase 10X Buffer

    C126A2 x 500μl
  • T4 DNA Ligase

    M180B1 x 500u
500u
(Weiss units)
1–3u/μl M1804 zł 412,00 Add to cart

T4 DNA Ligase (HC)

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T4 DNA Ligase (HC)

 Components

  • T4 DNA Ligase 10X Buffer

    C126B1 x 200μl
  • T4 DNA Ligase (HC)

    M179A1 x 500u
500u
(Weiss units)
10–20u/μl M1794 zł 412,00 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

T4 DNA Ligase Buffer Pack

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T4 DNA Ligase Buffer Pack

 Components

  • T4 DNA Ligase 10X Buffer

    C126A3 x 500μl
1.5ml
(3 × 500μl)
- C1263 zł 91,00 Add to cart

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QC Tests

Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.

Source

Recombinant E. coli strain.

Storage Buffer

10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

M1801, M1804, M1794 For Laboratory Use. Outside of the United States, this product is intended for research use only unless otherwise stated.
C1263 For Research Use Only. Not for Use in Diagnostic Procedures.

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