Promega Corporation

LigaFast™ Rapid DNA Ligation System

The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes). Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer. The LigaFast™ System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligation Buffer requires no additional ATP or Mg2+ addition prior to use.

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  • Prices valid for customers of Promega GmbH only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

LigaFast™ Rapid DNA Ligation System

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LigaFast™ Rapid DNA Ligation System

 Components

  • 2X Rapid Ligation Buffer

    C671A1 x 200μl
  • T4 DNA Ligase

    M180A1 x 100u
30 reactions
- M8221 zł 507,00 Add to cart

LigaFast™ Rapid DNA Ligation System

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LigaFast™ Rapid DNA Ligation System

 Components

  • 2X Rapid Ligation Buffer

    C671B2 x 500μl
  • T4 DNA Ligase

    M180B1 x 500u
150 reactions
- M8225 zł 2 085,00 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

2X Rapid Ligation Buffer

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2X Rapid Ligation Buffer

 Components

  • 2X Rapid Ligation Buffer

    C671B3 x 500μl
1.5ml
(3 × 500μl)
- C6711 Please Enquire

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QC Tests

Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay.

Storage Buffer

10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minutes.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

M8221, M8225, C6711 For Research Use Only. Not for Use in Diagnostic Procedures.

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