Promega Corporation

In Vitro Translation Specialty Vectors

The Flexi® Vector System is a simple, yet powerful, directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi® Vectors without the need to resequence.

The vectors below are designed with special sequences for maximal cell-free protein expression in a specific system. The pF3A/K WG vectors were designed for use with Wheat Germ extracts and contain sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, upstream a...

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pF3A WG (BYDV) Flexi® Vector

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pF3A WG (BYDV) Flexi® Vector

 Components

  • pF3A WG (BYDV) Flexi® Vector

    L567A1 x 20μg
20μg
- L5671 zł 1 266,00 Add to cart

pF3K WG (BYDV) Flexi® Vector

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pF3K WG (BYDV) Flexi® Vector

 Components

  • pF3K WG (BYDV) Flexi® Vector

    L568A1 x 20μg
20μg
- L5681 zł 1 266,00 Add to cart

pF25A ICE T7 Flexi® Vector

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pF25A ICE T7 Flexi® Vector

 Components

  • pF25A ICE T7 Flexi® Vector

    L106A1 x 20μg
20μg
- L1061 zł 1 266,00 Add to cart

pF25K ICE T7 Flexi® Vector

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pF25K ICE T7 Flexi® Vector

 Components

  • pF25K ICE T7 Flexi® Vector

    L108A1 x 20μg
20μg
- L1081 zł 1 266,00 Add to cart


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GenBank®/EMBL Accession Number

pF3A WG (BYDV) Flexi® Accession Number AY949043; pF3A WG (BYDV) Flexi® Vector sequence text file.
pF3K WG (BYDV) Flexi® Accession Number AY949044; pF3K WG (BYDV) Flexi® Vector sequence text file.
pF25A ICE T7 Flexi® Vector Accession Number EU754721; pF25A ICE T7 Flexi® Vector sequence text file.
pF25K ICE T7 Flexi® Vector Accession Number EU754722; pF25K ICE T7 Flexi® Vector sequence text file.

Storage Conditions

Store vectors at –20°C.

For product intended use please see Patents & Disclaimers tab.

pF3A WG (BYDV) Flexi Vector.Figure 1. pF3A WG (BYDV) Flexi® Vector.
pF3K WG (BYDV) Flexi Vector.Figure 2. pF3K WG (BYDV) Flexi® Vector.
pF25A ICE T7 Flexi Vector.Figure 3. pF25A ICE T7 Flexi® Vector.
pF25K ICE T7 Flexi Vector.Figure 4. pF25K ICE T7 Flexi® Vector.

Use Restrictions

L5671, L5681, L1061, L1081 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

L5671, L5681, L1061, L1081 European Pat. No. 1685247 and other patents pending.

L5671, L5681, L1061, L1081 Patent Pending.

L5671, L5681, L1061, L1081 For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

L5671, L5681 Licensed under U.S. Pat. No. 5,910,628.

L1061, L1081 Ezure, T., Suzuki, T., Higashide, S., Shintani, E., Endo, K., Kobayashi, S., Shikata, M., Ito, M., Tanimizu, K., Nishimura, O. (2006) Cell-free protein synthesis system prepared from insect cells by freeze-thawing. Biotechnol. Prog. 22, 1570–7.

L1061, L1081 Use of this vector for applications outside of the TNT® T7 Insect Cell Extract Protein Expression System may require a license from Shimadzu Corporation. For more information contact: t-direct@shimadzu-biotech.jp

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