Promega Corporation

Flexi® Cloning System

The Flexi® Vector System is a directional cloning method for protein-coding sequences. It is based on two rare-cutting restriction enzymes, SgfI and PmeI, and provides a rapid, efficient and high-fidelity way to transfer protein-coding regions without the need to resequence.

All Flexi® Vectors carry the lethal barnase gene, which is replaced by the DNA fragment of interest and acts as a positive selection for the successful ligation of the insert.

Unlike site-specific recombination vector systems, the Flexi® Vector Systems do not require appending multiple amino acids to the amino or carboxy termini of...

Expand to Read More »

  • Share
  • Print
  • Email
  • Prices valid for customers of Promega GmbH only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

Flexi® System, Entry/Transfer

Close Window

Flexi® System, Entry/Transfer

 Components

  • Wizard® SV Gel and PCR Clean-Up System

    A92801 x 10 preps
  • T4 DNA Ligase (HC)

    M179A1 x 500u
  • Nuclease-Free Water

    P119A1 x 1,250μl
  • 5X Flexi® Digest Buffer

    R186A1 x 1ml
  • 2X Flexi® Ligase Buffer

    R187A1 x 500μl
  • Flexi® Enzyme Blend (SgfI & PmeI)

    R188A2 x 50μl
5 entry and 20 transfer reactions
- C8640 zł 1 214,00 Add to cart

Flexi® System, Transfer

Close Window

Flexi® System, Transfer

 Components

  • T4 DNA Ligase (HC)

    M179A4 x 500u
  • Nuclease-Free Water

    P119A2 x 1,250μl
  • 5X Flexi® Digest Buffer

    R186A1 x 1ml
  • 2X Flexi® Ligase Buffer

    R187A2 x 500μl
  • Flexi® Enzyme Blend (SgfI & PmeI)

    R188A4 x 50μl
100 transfer reactions
- C8820 zł 3 750,00 Add to cart

Carboxy Flexi® System, Transfer

Close Window

Carboxy Flexi® System, Transfer

 Components

  • T4 DNA Ligase (HC)

    M179A2 x 500u
  • Nuclease-Free Water

    P119A1 x 1,250μl
  • 5X Flexi® Digest Buffer

    R186A1 x 1ml
  • 2X Flexi® Ligase Buffer

    R187A1 x 500μl
  • Flexi® Enzyme Blend (SgfI & PmeI)

    R188A1 x 50μl
  • Carboxy Flexi® Enzyme Blend (SgfI & EcoICRI)

    R190A1 x 50μl
50 transfer reactions
- C9320 zł 1 795,00 Add to cart

HaloTag® Cloning Starter System

Close Window

HaloTag® Cloning Starter System

 Components

  • Wizard® SV Gel and PCR Clean-Up System

    A92801 x 10 preps
  • Flexi® System, Entry/Transfer

    C86411 x 1 each
  • HaloTag® Flexi® Vectors—CMV Deletion Series Sample Pack

    G37801 x 9 × 2μg
  • Carboxy Flexi® Enzyme Blend (SgfI & EcoICRI)

    R19011 x 50μl
1 each
- G6050 zł 1 591,00 Add to cart


Available Separately

PRODUCT SIZE CONC CATALOG # *List Price ORDER QTY. Add to Cart

10X Flexi® Enzyme Blend (SgfI & PmeI)

Close Window

10X Flexi® Enzyme Blend (SgfI & PmeI)

 Components

  • Nuclease-Free Water

    P119A1 x 1,250μl
  • Flexi® Enzyme Blend (SgfI & PmeI)

    R185A1 x 25μl
  • 5X Flexi® Digest Buffer

    R186A1 x 1ml
25μl
- R1851 zł 407,00 Add to cart

10X Flexi® Enzyme Blend (SgfI & PmeI)

Close Window

10X Flexi® Enzyme Blend (SgfI & PmeI)

 Components

  • Nuclease-Free Water

    P119A2 x 1,250μl
  • 10X Flexi® Enzyme Blend (SgfI & PmeI)

    R185B1 x 100μl
  • 5X Flexi® Digest Buffer

    R186A1 x 1ml
100μl
- R1852 zł 1 405,00 Add to cart

Carboxy Flexi® Enzyme Blend (SgfI & EcoICRI)

Close Window

Carboxy Flexi® Enzyme Blend (SgfI & EcoICRI)

 Components

  • Nuclease-Free Water

    P119A1 x 1,250μl
  • 5X Flexi® Digest Buffer

    R186A1 x 1ml
  • Carboxy Flexi® Enzyme Blend (SgfI & EcoICRI)

    R190A1 x 50μl
50μl
- R1901 zł 364,00 Add to cart

You Also May Be Interested In

Storage Conditions

Cat.# C8640 is comprised of Cat.# C8641 and A9280. Store Cat.# C8641 at –20°C; store Cat.# A9280 at room temperature. Store Cat.# C8820 and C9320 at –20°C. Store enzyme blends at –20°C.

For product intended use please see Patents & Disclaimers tab.

Cloning a protein-coding region into the Flexi Vectors.Figure 1. Cloning a protein-coding region into the Flexi® Vectors.
Transfer of a protein-coding region between N-terminal or native Flexi® Vectors.Figure 2. Transfer of a protein-coding region between N-terminal or native Flexi® Vectors.

Use Restrictions

C8640, C8820, C9320, G6050, R1851, R1852, R1901 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G6050 BY USE OF THIS PRODUCT, RESEARCHER AGREES TO BE BOUND BY THE TERMS OF THIS LIMITED USE STATEMENT. If the researcher is not willing to accept the conditions of this limited use statement, and the product is unused, Promega will accept return of the unused product and provide the researcher with a full refund.
Researchers may use this product for research use only, no commercial use is allowed. Researchers shall have no right to modify or otherwise create variations of the nucleotide sequence of the HaloTag® gene. Researchers may however clone heterologous DNA sequences at either or both ends of said HaloTag® gene so as to create fused gene sequences provided that the coding sequence of the resulting HaloTag® gene has no more than four (4) deoxynucleotides missing at the affected terminus when compared to the intact HaloTag® gene sequence. In addition, researchers must do one of the following in conjunction with use of the product: (1) use Promega HaloTag® ligands, which can be modified or linked to Promega or customer-supplied moieties, or (2) contact Promega to obtain a license if Promega HaloTag® ligands are not to be used. Researchers may transfer derivatives to others for research use provided that at the time of transfer a copy of this label license is given to the recipients and recipients agree to be bound by the terms of this label license. With respect to any uses outside this label license, including any diagnostic, therapeutic or prophylactic uses, please contact Promega for supply and licensing information. PROMEGA MAKES NO REPRESENTATIONS OR WARRANTIES OF ANY KIND, EITHER EXPRESSED OR IMPLIED, INCLUDING FOR MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE WITH REGARDS TO THE PRODUCT. The terms of this agreement shall be governed under the laws of the State of Wisconsin, USA.

C8640, C8820, C9320, G6050, R1851, R1852, R1901 European Pat. No. 1685247 and other patents pending.

C8640, C8820, C9320, G6050 Patent Pending.

C8640, C8820, C9320, G6050, R1851, R1852, R1901 U.S. Pat. No. 5,391,487.

G6050 U.S. Pat. Nos. 7,425,436 and 7,935,803 and other patents pending.

C8640, C8820, C9320, G6050 For research use only. Persons wishing to use this product or its derivatives in other fields of use, including without limitation, commercial sale, diagnostics or therapeutics, should contact Promega Corporation for licensing information.

C8640, C8820, C9320, G6050, R1851, R1852 Licensed under U.S. Pat. No. 5,945,288.

Prefer a different language?

Your country is set to Poland. Your language is set to polski. Please select the language that will best suit your needs:

This is correct, continue to site »

I need additional help

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.