Promega Corporation

Exonuclease III

Exonuclease III is a 3´→5´ exonuclease specific for double-stranded DNA. The enzyme catalyzes the stepwise removal of mononucleotides starting from a 3´-OH at nicks, blunt ends, recessed ends and 3´-overhangs of less than 4 bases, yielding nucleoside 5´-phosphates. Exonuclease III will also degrade DNA from 3´-phosphate ends due to its intrinsic 3´-phosphatase activity. In addition, the enzyme has apurinic endonuclease activity and ribonuclease H activity. Exonuclease III is used in conjunction with S1 nuclease for unidirectional deletion of sequences from the termini of DNA fragments.

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Exonuclease III

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Exonuclease III


  • Exonuclease III 10X Buffer

    E577B1 x 1ml
  • Exonuclease III

    M181A1 x 5,000u
150–200u/μl M1811 zł 295,00 Add to cart

Exonuclease III

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Exonuclease III


  • Exonuclease III 10X Buffer

    E577B5 x 1ml
  • Exonuclease III

    M181C1 x 25,000u
150–200u/μl M1815 zł 1 058,00 Add to cart

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QC Tests

Activity, SDS-PAGE/purity, endonuclease, 3´ overhang protection assay.


Recombinant E. coli strain.

Storage Buffer

20mM Tris-HCl (pH 7.5 at 25°C), 1mM DTT, 100mM KCl, 50% (v/v) glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme required to produce 1nmol of acid-soluble nucleotides from double-stranded DNA in 30 minutes at 37°C.

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

M1811, M1815 For Research Use Only. Not for Use in Diagnostic Procedures.

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