Promega Corporation

DNA Polymerase I

DNA Polymerase I catalyzes the template-directed polymerization of nucleotides into duplex DNA in a 5´→3´ direction. DNA Polymerase I possesses a 3´→5´ exonuclease activity or "proofreading" function, which lowers the error rate during DNA replication, and also contains a 5´→3´ exonuclease activity, which enables the enzyme to replace nucleotides in the growing strand of DNA by nick translation. The enzyme, purified from recombinant E. coli, is capable of catalyzing de novo synthesis of synthetic homopolymers and provides a convenient method for the preparation of a variety of defined DNA substrates.

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DNA Polymerase I

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DNA Polymerase I

 Components

  • DNA Polymerase 10X Buffer

    M195A1 x 1ml
  • DNA Polymerase I

    M205A1 x 500u
500u
5–10u/μl M2051 zł 399,00 Add to cart

DNA Polymerase I

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DNA Polymerase I

 Components

  • DNA Polymerase 10X Buffer

    M195A5 x 1ml
  • DNA Polymerase I

    M205B1 x 2,500u
2,500u
5–10u/μl M2055 zł 1 639,00 Add to cart


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QC Tests

Activity, SDS-PAGE/purity, endonuclease.

Source

Recombinant E. coli strain.

Storage Buffer

50mM Tris-HCl (pH 7.5 at 25°C), 1mM DTT, 0.1mM EDTA, 50% (v/v) glycerol.

Storage Conditions

Store at –20°C.

Unit Definition

One unit is defined as the amount of enzyme that incorporates 10nmol of deoxyribonucleotides into TCA-insoluble material in 30 minutes at 37°C. The reaction conditions are: 67mM potassium phosphate (pH 7.4 at 25°C), 6.7mM MgCl2, 1mM DTT, 50μg/ml activated calf thymus DNA and 33μM dATP, dCTP, dGTP and dTTP (a mix of unlabeled and [3H]dTTP).

For product intended use please see Patents & Disclaimers tab.

Use Restrictions

M2051, M2055 For Research Use Only. Not for Use in Diagnostic Procedures.

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