Alkaline Phosphatase, Calf Intestinal (CIAP)

M1821_Alkaline-Phosphatase-Calf-Intestinal_3
View information on global supply logistics
customize-this-small

CIAP catalyzes the Hydrolysis of 5´ Phosphate Groups from DNA, RNA, and r- and dNTPs

  • Available at high concentration; Cat.# M2825 contains 1,000 units of CIAP at 20u/μl
  • Qualified for blue/white cloning; our assay provides a higher level of quality control for enzymes used in cloning applications

Choose a concentration

Size

Catalog number selected: M1821

$ 96.00
Your price:
Add to Cart
This product is discontinued
Add to Helix
This product is available under our Early Access program - Learn More
This product is available under our Catalog (FT) program - Learn More
Alkaline Phosphatase, Calf Intestinal (CIAP)
1u/µl/1,000u
$ 96.00
Your price: Log in
Change Configuration

Alkaline Phosphatase Product Details

Alkaline Phosphatase, Calf Intestinal (CIAP), catalyzes the hydrolysis of 5´-phosphate groups from nucleic acids and nucleotide triphosphates. This enzyme is used to prevent recircularization and religation of linearized cloning vector DNA by removing phosphate groups from both 5´-termini and may also be used for the dephosphorylation of 5´ phosphorylated ends of DNA or RNA for subsequent labeling with [32P]ATP and T4 Polynucleotide Kinase. CIAP is active on 5´ overhangs, 5´ recessed and blunt ends. CIAP is provided with 10X Reaction Buffer: 0.5M Tris-HCl (pH 9.3 at 25°C), 10mM MgCl2, 1mM ZnCl2, 10mM spermidine.

Applications

  • Preventing religation of linearized cloning vehicle DNA by removing phosphate groups from both 5´ termini.
  • Removing 5´ phosphate groups prior to end-labeling with T4 Polynucleotide Kinase.

References

  1. Sambrook, J. et al. (1989) Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
  2. Seeburg, P.H. et al. (1977) Nature 270, 486–94.
  3. Ullrich, A. et al. (1977) Science 196, 1313–9.
  4. Meyerowitz, E.M. et al. (1980) Gene 11, 271–82.
  5. Grosveld, F.G. et al. (1981) Gene 13, 227–37.

Storage Buffer: 10mM Tris-HCl (pH 8.0), 1mM MgCl2, 0.1mM ZnCl2, 50mM KCl, 50% (v/v) glycerol.

Source: Calf intestinal mucosa.

QC Tests: Activity, endonuclease, DNase, RNase, blue/white cloning assay.

Unit Definition: One unit is defined as the amount of enzyme required to catalyze the hydrolysis of 1μmol of 4-nitrophenyl phosphate per minute at 37°C in 1M diethanolamine, 10.9mM 4-nitrophenyl phosphate, 0.5mM MgCl2 (pH 9.8 at 25°C).

Specifications

You are viewing: M1821 Change Configuration

What's in the box?

Item Part # Size Available Separately

Alkaline Phosphatase, Calf Intestinal

M182A 1 × 1,000u

Alkaline Phosphatase 10X Buffer

M183A 2 × 500μl View Product

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Specifications

You are viewing: M2825 Change Configuration

What's in the box?

Item Part # Size Available Separately

Alkaline Phosphatase 10X Buffer

M183A 2 × 500μl View Product

Alkaline Phosphatase Calf Intestinal (HC)

M282A 1 × 1,000u

Certificate of Analysis

Search by lot number

Use Restrictions

For Research Use Only. Not for Use in Diagnostic Procedures.

Storage Conditions

BB

Let's find the product that meets your needs.

Talk to a Scientist

Luca

Luca

Italy