Promega Corporation

Kinase-Glo® Luminescent Kinase Assays

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The Kinase-Glo® Luminescent Kinase Assays are homogeneous non-radioactive methods for determining the activity of purified kinases by quantifying the amount of ATP remaining in solution following a kinase reaction. The assays are designed for use with multiwell plate formats, making them ideal for automated high-throughput screening (HTS), and they can be used to assay protein, lipid and sugar kinases. The assay procedure involves addition of a single reagent directly to a completed kinase reaction. This addition results in the generation of a luminescent signal correlated with the amount of ATP present and invers...

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Kinase-Glo® Luminescent Kinase Assay

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Kinase-Glo® Luminescent Kinase Assay

 Components

  • Kinase-Glo® Substrate

    V672A1 x 1 vial
  • Kinase-Glo® Buffer

    V673A1 x 10ml
10ml
- V6711 zł 403,00 Add to cart

Kinase-Glo® Luminescent Kinase Assay

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Kinase-Glo® Luminescent Kinase Assay

 Components

  • Kinase-Glo® Substrate

    V672A10 x 1 vial
  • Kinase-Glo® Buffer

    V673A10 x 10ml
10 × 10ml
- V6712 zł 1 413,00 Add to cart

Kinase-Glo® Luminescent Kinase Assay

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Kinase-Glo® Luminescent Kinase Assay

 Components

  • Kinase-Glo® Substrate

    V672B1 x 1 vial
  • Kinase-Glo® Buffer

    V673B1 x 100ml
100ml
- V6713 zł 1 301,00 Add to cart

Kinase-Glo® Luminescent Kinase Assay

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Kinase-Glo® Luminescent Kinase Assay

 Components

  • Kinase-Glo® Substrate

    V672B10 x 1 vial
  • Kinase-Glo® Buffer

    V673B10 x 100ml
10 × 100ml
- V6714 Please Enquire

Kinase-Glo® Max Luminescent Kinase Assay

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Kinase-Glo® Max Luminescent Kinase Assay

 Components

  • Kinase-Glo® Max Buffer

    V608A1 x 10ml
  • Kinase-Glo® Max Substrate

    V609A1 x Resuspend to 10ml
10ml
- V6071 zł 434,00 Add to cart

Kinase-Glo® Max Luminescent Kinase Assay

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Kinase-Glo® Max Luminescent Kinase Assay

 Components

  • Kinase-Glo® Max Buffer

    V608A10 x 10ml
  • Kinase-Glo® Max Substrate

    V609A10 x Resuspend to 10ml
10 × 10ml
- V6072 zł 1 812,00 Add to cart

Kinase-Glo® Max Luminescent Kinase Assay

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Kinase-Glo® Max Luminescent Kinase Assay

 Components

  • Kinase-Glo® Max Buffer

    V608B1 x 100ml
  • Kinase-Glo® Max Substrate

    V609B1 x Resuspend to 100ml
100ml
- V6073 zł 1 673,00 Add to cart

Kinase-Glo® Max Luminescent Kinase Assay

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Kinase-Glo® Max Luminescent Kinase Assay

 Components

  • Kinase-Glo® Max Buffer

    V608B10 x 100ml
  • Kinase-Glo® Max Substrate

    V609B10 x Resuspend to 100ml
10 × 100ml
- V6074 Please Enquire

Kinase-Glo® Plus Luminescent Kinase Assay

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Kinase-Glo® Plus Luminescent Kinase Assay

 Components

  • Kinase-Glo® Plus Substrate

    V378A1 x Lyophilized, Resuspend to 10ml
  • Kinase-Glo® Plus Buffer

    V379A1 x 10ml
10ml
- V3771 zł 416,00 Add to cart

Kinase-Glo® Plus Luminescent Kinase Assay

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Kinase-Glo® Plus Luminescent Kinase Assay

 Components

  • Kinase-Glo® Plus Substrate

    V378A10 x Lyophilized, Resuspend to 10ml
  • Kinase-Glo® Plus Buffer

    V379A10 x 10ml
10 × 10ml
- V3772 zł 1 738,00 Add to cart

Kinase-Glo® Plus Luminescent Kinase Assay

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Kinase-Glo® Plus Luminescent Kinase Assay

 Components

  • Kinase-Glo® Plus Substrate

    V378B1 x Lyophilized, Resuspend to 100ml
  • Kinase-Glo® Plus Buffer

    V379B1 x 100ml
100ml
- V3773 zł 1 600,00 Add to cart

Kinase-Glo® Plus Luminescent Kinase Assay

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Kinase-Glo® Plus Luminescent Kinase Assay

 Components

  • Kinase-Glo® Plus Substrate

    V378B10 x Lyophilized, Resuspend to 100ml
  • Kinase-Glo® Plus Buffer

    V379B10 x 100ml
10 × 100ml
- V3774 Please Enquire


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Storage Conditions

Store at –20°C. Avoid multiple freeze-thaw cycles and exposure to frequent temperature changes. These fluctuations can greatly alter product stability.

For product intended use please see Patents & Disclaimers tab.

Luminescent output correlates with amount of ATP.Figure 1. Luminescent output correlates with amount of ATP.

A direct relationship exists between the luminescence measured with the Kinase-Glo® Assays and the amount of ATP in the reaction. Panel A. Kinase-Glo® Plus Assay. Panel B. Kinase-Glo® Max Assay. The Kinase-Glo® Assay is linear to 10µM (data not shown).

Use Restrictions

V6711, V6712, V6713, V6714, V6071, V6072, V6073, V6074, V3771, V3772, V3773, V3774 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

V6711, V6712, V6713, V6714, V6071, V6072, V6073, V6074, V3771, V3772, V3773, V3774 U.S. Pat. Nos. 7,083,911, 7,452,663 and 7,732,128 and other patents.

V6711, V6712, V6713, V6714, V6071, V6072, V6073, V6074, V3771, V3772, V3773, V3774 U.S. Pat. No. 7,700,310 and other patents and patents pending.

V6711, V6712, V6713, V6714, V6071, V6072, V6073, V6074, V3771, V3772, V3773, V3774 U.S. Pat. No. 7,741,067 and other patents and patents pending.

V6711, V6712, V6713, V6714, V6071, V6072, V6073, V6074, V3771, V3772, V3773, V3774 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

V6711, V6712, V6713, V6714, V6071, V6072, V6073, V6074, V3771, V3772, V3773, V3774 Licensed from Lonza Nottingham Ltd. under U.S. Pat. Nos. 6,599,711 and 6,911,319 and other pending and issued patents.

V6711, V6712, V6713, V6714, V6071, V6072, V6073, V6074, V3771, V3772, V3773, V3774 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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