The HDAC-Glo™ and SIRT-Glo™ Assays and Screening Systems provide a simple "Add-Mix-Measure" method that achieves maximum signal in as little as 15 minutes and is 10-to 100X more sensitive than comparable fluorescence-based systems.
HDAC-Glo™ I/II Assays
Measure histone deacetylation activity in cells, extracts or from purified enzyme sources with these simple, “Add-Mix-Measure” luminescent assays and screening systems.
SIRT-Glo™ Assays
Single-reagent-addition, homogeneous, luminescent assays and screening systems that measure the relative activity of the NAD+-dependent histone deacetylase class III enzymes (sirtuins; SIRTs) from purified enzyme sources.
Here we describe efforts to define the utility of the HDAC-Glo™ Assay using structurally diverse HDAC inhibitors and various cell lines.
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