CellTiter-Glo® Luminescent Cell Viability Assay

The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. The CellTiter-Glo® Assay is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays. The homogeneous assay procedure involves adding the single reagent (CellTiter-Glo® Reagent) directly to cells cultured in serum-supplemented medium. Cell washing, removal of medium and multiple pipetting steps are not required. Th...

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The CellTiter-Glo® Luminescent Cell Viability Assay is a homogeneous method of determining the number of viable cells in culture based on quantitation of the ATP present, an indicator of metabolically active cells. The CellTiter-Glo® Assay is designed for use with multiwell formats, making it ideal for automated high-throughput screening (HTS), cell proliferation and cytotoxicity assays. The homogeneous assay procedure involves adding the single reagent (CellTiter-Glo® Reagent) directly to cells cultured in serum-supplemented medium. Cell washing, removal of medium and multiple pipetting steps are not required. The system detects as few as 15 cells/well in a 384-well format in 10 minutes after adding reagent and mixing.

The homogeneous "add-mix-measure" format results in cell lysis and generation of a luminescent signal proportional to the amount of ATP present. The amount of ATP is directly proportional to the number of cells present in culture. The CellTiter-Glo® Assay generates a "glow-type" luminescent signal, which has a half-life generally greater than five hours, depending on cell type and medium used. The extended half-life eliminates the need to use reagent injectors and provides flexibility for continuous or batch mode processing of multiple plates. The unique homogeneous format avoids errors that may be introduced by other ATP measurement methods that require multiple steps.

Features - Benefits

• Simplify Cell Viability Assays: Homogeneous "add-mix-measure" format dramatically reduces the number of plate handling steps required for similar assays.
• Use Fewer Cells: Detects as few as 15 cells/well in a 384-well format or 50 cells/well in a 96-well format. Accurately measures cells at numbers below the detection limits of standard colorimetric and fluorometric assays. Reduces the number of cells required per assay.
• Get Results Quickly: Data can be recorded 10 minutes after adding reagent.
• Choose Your Format: Can be used with various multiwell formats. Data can be recorded by luminometer or CCD camera imaging device.
• Process Plates Consecutively: Luminescent signal is very stable, with a half-life generally >5 hours, dependent on cell type and medium used, allowing batch processing; delivers excellent Z´-factor values for screening applications.
• Get More Information: Multiplex with other cell-based assays from Promega.
• Automate This Assay: Validated automated methods available at: www.promega.com/automethods/
• Choose Your Configuration: Learn more about our custom options for this product at: www.promega.com/myway/

Applications

• Cell proliferation.
• Cytotoxicity.
• Cell viability.

For more information, see the Protocols & Applications Guide.

Notes

Using 100μl of CellTiter-Glo® Reagent per assay in a 96-well format, Cat.# G7570 is sufficient to perform 100 assays; Cat.# G7571 and G7572, 1,000 assays; Cat.# G7573, 10,000 assays. Using 25μl of CellTiter-Glo® Reagent per assay in a 384-well format, Cat.# G7570 is sufficient to perform 400 assays; Cat.# G7571 and G7572, 4,000 assays; Cat.# G7573, 40,000 assays.

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