Promega Corporation

CytoTox-Glo™ Cytotoxicity Assay

The CytoTox-Glo™ Assay is a luminescent cytotoxicity assay that measures the relative number of dead cells in cell populations. The CytoTox-Glo™ Assay measures the extracellular activity of a distinct intracellular protease activity (dead-cell protease) when the protease is released from membrane-compromised cells. A luminogenic cell-impermeant peptide substrate (AAF-aminoluciferin) is used to measure dead-cell protease activity. The liberated aminoluciferin product is measured as "glow type" luminescence generated by Ultra-Glo™ Recombinant Luciferase provided in the assay reagent. The AAF-aminoluciferin substrate...

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CytoTox-Glo™ Cytotoxicity Assay

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CytoTox-Glo™ Cytotoxicity Assay

 Components

  • Assay Buffer

    G146A2 x 5ml
  • AAF-Glo™ Substrate

    G607A1 x 2ml
  • Digitonin

    G944A1 x 40μl
10ml
- G9290 zł 407,00 Add to cart

CytoTox-Glo™ Cytotoxicity Assay

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CytoTox-Glo™ Cytotoxicity Assay

 Components

  • Assay Buffer

    G146A10 x 5ml
  • AAF-Glo™ Substrate

    G607A5 x 2ml
  • Digitonin

    G944B1 x 175μl
5 × 10ml
- G9291 zł 1 777,00 Add to cart

CytoTox-Glo™ Cytotoxicity Assay

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CytoTox-Glo™ Cytotoxicity Assay

 Components

  • Assay Buffer

    G146B4 x 25ml
  • AAF-Glo™ Substrate

    G607B2 x 10ml
  • Digitonin

    G944B2 x 175μl
2 × 50ml
- G9292 zł 3 477,00 Add to cart


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Storage Conditions

Store at –20°C, protected from light.

For product intended use please see Patents & Disclaimers tab.

Cleavage of the luminogenic AAF-Glo Substrate by dead-cell protease activity.Figure 1. Cleavage of the luminogenic AAF-Glo™ Substrate by dead-cell protease activity.

Following cleavage, a substrate for luciferase (aminoluciferin) is released, resulting in the luciferase-mediated production of light.

The CytoTox-Glo Cytotoxicity Assay shows strong correlation with established methods for measuring cytotoxicity.Figure 2. The CytoTox-Glo™ Cytotoxicity Assay shows strong correlation with established methods for measuring cytotoxicity.
Overview of the CytoTox-Glo Cytotoxicity Assay protocol.Figure 3. Overview of the CytoTox-Glo™ Cytotoxicity Assay protocol.

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Superior sensitivity and dynamic range compared to fluorescent LDH assay.Figure 4. Superior sensitivity and dynamic range compared to fluorescent LDH assay.

Use Restrictions

G9290, G9291, G9292 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G9290, G9291, G9292 Patent Pending.

G9290, G9291, G9292 U.S. Pat. Nos. 6,602,677, 7,241,584 and 8,030,017 and other patents and patents pending.

G9290, G9291, G9292 The method of recombinant expression of Coleoptera luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.

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