Promega Corporation

CellTox™ Green Cytotoxicity Assay

The CellTox™ Green Cytotoxicity Assay measures changes in membrane integrity that occur as a result of cell death. The assay is intended to assess cytotoxicity in cell culture after experimental manipulation. The assay system uses a proprietary asymmetric cyanine dye that is excluded from viable cells but preferentially stains the DNA from dead cells. When the dye binds DNA released from cells, its fluorescence properties are substantially enhanced. Viable cells produce no appreciable increases in fluorescence. Therefore, the fluorescence signal produced by the binding interaction with dead cell DNA is prop...

Expand to Read More »

  • Share
  • Print
  • Email
  • Prices valid for customers of Promega GmbH only
Product Size Conc. Catalog # *List Price Order QTY Add to Cart

CellTox™ Green Cytotoxicity Assay

Close Window

CellTox™ Green Cytotoxicity Assay

 Components

  • Lysis Solution

    G182B1 x 0.5ml
  • Assay Buffer

    G610A1 x 10ml
  • CellTox™ Green Dye, 1,000X

    G873A1 x 20μl
10ml
- G8741 zł 295,00 Add to cart

CellTox™ Green Cytotoxicity Assay

Close Window

CellTox™ Green Cytotoxicity Assay

 Components

  • Lysis Solution

    G182B1 x 0.5ml
  • Assay Buffer

    G610B1 x 50ml
  • CellTox™ Green Dye, 1,000X

    G873A5 x 20μl
50ml
- G8742 zł 1 188,00 Add to cart

CellTox™ Green Cytotoxicity Assay

Close Window

CellTox™ Green Cytotoxicity Assay

 Components

  • Lysis Solution

    G182B1 x 0.5ml
  • Assay Buffer

    G610B2 x 50ml
  • CellTox™ Green Dye, 1,000X

    G873B1 x 200μl
100ml
- G8743 zł 2 081,00 Add to cart

CellTox™ Green Express Cytotoxicity Assay

Close Window

CellTox™ Green Express Cytotoxicity Assay

 Components

  • CellTox™ Green Dye, 1,000X

    G873B1 x 200μl
200μl
- G8731 zł 1 821,00 Add to cart


You Also May Be Interested In

Storage Conditions

Store at –20°C.

For product intended use please see Patents & Disclaimers tab.

10887MA_new_582pxFigure 1. CellTox™ Green Dye binds DNA of cells with impaired membrane integrity.

/~/media/images/resources/figures/10800-10899/10887ma_new_582px.jpg?la=pl-PL
CellTox™ Green Dye fluorescence is proportional to dead-cell number.Figure 2. CellTox™ Green Dye fluorescence is proportional to dead-cell number.
Schematic diagram of methods for delivering the CellTox™ Green Dye.Figure 3. Schematic diagram of methods for delivering the CellTox™ Green Dye.

/~/media/images/resources/figures/10800-10899/10888ma_1140px.jpg?la=pl-PL
Cytotoxicity is concentration- and exposure period-dependent.Figure 4. Cytotoxicity is concentration- and exposure period-dependent.

Staurosporine was applied to K562 cells after introducing the CellTox™ Green Dye following the Express, No-Step Addition Method at Seeding Protocol. Fluorescence associated with the progression of cytotoxicity was measured after 24, 48 and 72 hours of cell exposure.

Multiplexing allows complementary measures of cell health.Figure 5. Multiplexing allows complementary measures of cell health.

CellTox™ Green Reagent was applied to bortezomib-treated K562 cells after 48 hours of exposure. Fluorescence associated with cytotoxicity was measured, then CellTiter-Glo® Reagent applied and bioluminescence associated with viability measured. These inverse measures produce similar EC50 values.

CellTox™ Green Reagent was applied to bortezomib-treated K562 cells after 48 hours of exposure. Fluorescence associated with cytotoxicity was measured, then CellTiter-Glo® Reagent applied and bioluminescence associated with viability measured. These inverse measures produce similar EC50 values.

/~/media/images/resources/figures/10900-10999/10935ma_524px.jpg?la=pl-PL

Use Restrictions

G8741, G8742, G8743, G8731 For Research Use Only. Not for Use in Diagnostic Procedures.

Patents - Disclaimers

G8741, G8742, G8743, G8731 Patent Pending.

Prefer a different language?

Your country is set to Poland. Your language is set to polski. Please select the language that will best suit your needs:

This is correct, continue to site »

I need additional help

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.